LOCUS       sequence-114391-         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Plasma Membrane, Excitation: 604, Emission: 659.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Chu J, Haynes RD, Corbel SY, Li P, Gonzalez-Gonzalez E, Burg JS, 
            Ataie NJ, Lam AJ, Cranfill PJ, Baird MA, Davidson MW, Ng HL, Garcia 
            KC, Contag CH, Shen K, Blau HM, Lin MZ
  TITLE     Non-invasive intravital imaging of cellular differentiation with a 
            bright red-excitable fluorescent protein.
  JOURNAL   Nat Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888.
  PUBMED    24633408
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888."
COMMENT     Sequence Label: mCardinal-Farnesyl-5
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      35..44
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             45..773
                     /codon_start=1
                     /product="far-red fluorescent protein derived from 
                     mNeptune, with further red-shifted spectra"
                     /label=mCardinal
                     /translation="VSKGEELIKENMHMKLYMEGTVNNHHFKCTTEGEGKPYEGTQTQR
                     IKVVEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGV
                     LTVTQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEATTETLYPADGGLEGRCDM
                     ALKLVGGGHLHCNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADNETYVEQHEVAVA
                     RYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 aaccggtcag aatccgctta gcgctaccgg ggtcgccacc atgggtgagc aagggcgagg
       61 agctgatcaa ggagaacatg cacatgaagc tgtacatgga aggcaccgtg aacaaccacc
      121 acttcaagtg caccaccgaa ggggagggca agccctacga gggcacccag acccagagga
      181 ttaaggtggt ggagggaggc cccctgccgt tcgcattcga catcctggcc acctgcttta
      241 tgtacgggag caagaccttc atcaaccaca cccagggcat ccccgatttc tttaagcagt
      301 ccttccctga gggcttcaca tgggagagag tcaccacata cgaagacggg ggcgtgctta
      361 ccgttaccca ggacaccagc ctccaggacg gctgcttgat ctacaacgtc aagctcagag
      421 gggtgaactt cccatccaac ggccctgtga tgcagaagaa aacactcggc tgggaggcca
      481 ccaccgagac cctgtacccc gctgacggcg gcctggaagg cagatgcgac atggccctga
      541 agctcgtggg cgggggccac ctgcactgca acctgaagac cacatacaga tccaagaaac
      601 ccgctaagaa cctcaagatg cccggcgtct actttgtgga ccgcagactg gaaagaatca
      661 aggaggccga caatgagacc tacgtcgagc agcacgaggt ggctgtggcc agatactgcg
      721 acctccctag caaactgggg cacaaactta atggcatgga cgagctgtac aagtccggac
      781 tcagatctaa gctgaaccct cctgatgaga gtggccccgg ctgcatgagc tgcaagtgtg
      841 tgctctcctg agaattctgc agtcgacggt
//
