LOCUS       sequence-114490-         738 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Endoplasmic reticulum (ER)-targeted FRET-based PKA activity 
            reporter; targeted to cytosolic face of ER membrane..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Mehta S, Aye-Han NN, Ganesan A, Oldach L, Gorshkov K, Zhang J
  TITLE     Calmodulin-controlled spatial decoding of oscillatory Ca2+ signals 
            by calcineurin.
  JOURNAL   eLife. 2014 Jul 23;3:e03765. doi: 10.7554/eLife.03765.
  PUBMED    25056880
REFERENCE   2  (bases 1 to 738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2014-07-23"; volume: "3:e03765. doi"; pages: " 
            10.7554/eLife.03765"
COMMENT     Sequence Label: pcDNA3-ER-AKAR4
FEATURES             Location/Qualifiers
     source          1..738
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     440..461
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(567..588)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 tactactagg agaagacggt aaccttttat tgaatgacat ttccactaat gggacctggt
       61 taaatgggca aaaagtcgag aagaacagca atcagttact gtctcaaggt gatgaaataa
      121 ccgttggtgt aggcgtggaa tcagatattt tatctctggt cattttcata aacgacaaat
      181 ttaagcagtg cctcgagcag aacaaagttg atcgctctgc aggtaagcca ggcagcggcg
      241 agggcagcac caagggcctg cgtcgcgcca ccctggtgga cggcggcacc ggcggcagcg
      301 agctcatggg cggcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
      361 gccccgtgct gctgcccgac aaccactacc tgagctacca gtccaagctg agcaaagacc
      421 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
      481 tcggcatgga cgagctgtac aagggaggta ccggtggatc tatggtgagc aagggcgagg
      541 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
      601 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagc
      661 tgatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgggct
      721 acggccttca gtgcttcg
//
