LOCUS       sequence-114539-         732 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Chen Y, Partow S, Scalcinati G, Siewers V, Nielsen J
  TITLE     Enhancing the copy number of episomal plasmids in Saccharomyces 
            cerevisiae for improved protein production.
  JOURNAL   FEMS Yeast Res. 2012 Aug;12(5):598-607. doi: 
            10.1111/j.1567-1364.2012.00809.x. Epub 2012 Apr 25.
  PUBMED    22487308
REFERENCE   2  (bases 1 to 732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1567-1364.2012.00809.x"; journalName: "FEMS Yeast Res"; 
            date: "2012-08"; volume: "12"; issue: "5"; pages: "598-607"
COMMENT     Sequence Label: pSP-GM2
FEATURES             Location/Qualifiers
     source          1..732
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(60..249)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     CDS             complement(279..308)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(325..344)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(326..344)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcgcgttggc cgattcatta atgcagctgg ataaacgccg gcgaaacgat ccggaggatc
       61 ttcgagcgtc ccaaaacctt ctcaagcaag gttttcagta taatgttaca tgcgtacacg
      121 cgtctgtaca gaaaaaaaag aaaaatttga aatataaata acgttcttaa tactaacata
      181 actataaaaa aataaatagg gacctagact tcaggttgtc taactccttc cttttcggtt
      241 agagcggatc ttagctagcc gcggtaccaa gcttactcga ggtcttcttc ggaaatcaac
      301 ttctgttcca tgtcgacgcc cgggccctat agtgagtcgt attacggatc cttgtaatta
      361 aaacttagat tagattgcta tgctttcttt ctaatgagca agaagtaaaa aaagttgtaa
      421 tagaacaaga aaaatgaaac tgaaacttga gaaattgaag accgtttatt aacttaaata
      481 tcaatgggag gtcatcgaaa gagaaaaaaa tcaaaaaaaa aaattttcaa gaaaaagaaa
      541 cgtgataaaa atttttattg cctttttcga cgaagaaaaa gaaacgaggc ggtctctttt
      601 ttcttttcca aacctttagt acgggtaatt aacgacaccc tagaggaaga aagaggggaa
      661 atttagtatg ctgtgcttgg gtgttttgaa gtggtacggc gatgcgcgga gtccgagaaa
      721 atctggaaga gt
//
