LOCUS       sequence-114598-         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Ras GTPase, Excitation: 448 / 473, Emission: 500 / 
            528.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Shu X, Lev-Ram V, Deerinck TJ, Qi Y, Ramko EB, Davidson MW, Jin Y, 
            Ellisman MH, Tsien RY
  TITLE     A genetically encoded tag for correlated light and electron 
            microscopy of intact cells, tissues, and organisms.
  JOURNAL   PLoS Biol. 2011 Apr;9(4):e1001041. doi: 
            10.1371/journal.pbio.1001041. Epub 2011 Apr 5.
  PUBMED    21483721
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2011-04"; volume: "9(4):e1001041. doi"; pages: " 
            10.1371/journal.pbio.1001041. Epub 2011 Apr 5"
COMMENT     Sequence Label: miniSOG-Rab5a-7
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..349
                     /codon_start=1
                     /product="mini singlet oxygen generator, a fluorescent 
                     flavoprotein suitable for correlated light and electron 
                     microscopy (Shu et al., 2011)"
                     /label=miniSOG
                     /translation="MEKSFVITDPRLPDNPIIFASDGFLELTEYSREEILGRNGRFLQG
                     PETDQATVQKIRDAIRDQREITVQLINYTKSGKKFWNLLHLQPMRDQKGELQYFIGVQL
                     DG"
ORIGIN
        1 gtcagatccg ctagcgctac cggtcgccac catggagaaa agtttcgtga taactgatcc
       61 acggctgcca gacaatccca tcatcttcgc atccgatggc ttcctggagc tgaccgagta
      121 ttccagagag gagatcctgg gccgcaatgg ccgctttctg cagggaccag agacagacca
      181 ggccacagtg cagaagattc gcgatgccat tagagatcag cgcgagatta ccgtgcagct
      241 gataaactac acaaaaagcg ggaagaaatt ctggaacctc ctgcacctcc agcccatgag
      301 ggaccagaag ggtgagctcc agtatttcat cggagtgcag ctggatggat ccggactcag
      361 atctcgagcc atggctagtc gaggcgcaac aagacccaac gggccaaata ctggaaataa
      421 aatatgccag ttcaaactag tacttctggg agagtctgct gttggcaaat caagcctagt
      481 gcttcgtttt gtgaagggcc aatttcatga atttcaagag agtaccatag gggctgcttt
      541 tctaacccaa actgtgtgtc ttgatgatac aacagtaaag tttgaaatat gggatacagc
      601 tggtcaagaa cgataccata gcttagcacc aatgtactac agaggagcac aagcagccat
      661 agttgtatat gatatcacaa atgaggagtc ctttgccaga gccaaaaact gggttaaaga
      721 acttcagagg caagccagtc ctaacattgt aatagcttta tcaggaaaca aggctgatct
      781 tgcaaataaa agagctgtcg atttccagga agcacagtcc tatgcagatg acaacagttt
      841 attattcatg gagacatcag ctaaaacatc
//
