LOCUS       sequence-114600-         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-Sec61-N-18
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             24..311
                     /codon_start=1
                     /gene="SEC61B"
                     /product="human Sec61-beta subunit of the translocon in the
                     endoplasmic reticulum"
                     /label=Sec61-beta
                     /translation="MPGPTPSGTNVGSSGRSPSKAVAARAAGSTVRQRKNASCGTRSAG
                     RTTSAGTGGMWRFYTEDSPGLKVGPVPVLVMSLLFIASVFMLHIWGKYTRS"
     regulatory      360..369
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(511..529)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     779..798
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ccgtcagatc cgctagcgcc accatgcctg gtccgacccc cagtggcact aacgtgggat
       61 cctcagggcg ctctcccagc aaagcagtgg ccgcccgggc ggcgggatcc actgtccggc
      121 agaggaaaaa tgccagctgt gggacaagga gtgcaggccg cacaacctcg gcaggcaccg
      181 gggggatgtg gcgattctac acagaagatt cacctgggct caaagttggc cctgttccag
      241 tattggttat gagtcttctg ttcatcgctt ctgtatttat gttgcacatt tggggcaagt
      301 acactcgttc gggacgaatt ctgcagtcga cggtaccgcg ggcccgggat ccaccggtcg
      361 ccaccatggt gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct
      421 tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg
      481 agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc
      541 tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga
      601 agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg
      661 agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc
      721 aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc
      781 ccgtaatgca gaagaagacc atgggctggg aggcctcctc cga
//
