LOCUS       sequence-114627-         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plum eIF4E ihp-RNA construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Wang X, Kohalmi SE, Svircev A, Wang A, Sanfacon H, Tian L
  TITLE     Silencing of the host factor eIF(iso)4E gene confers plum pox virus 
            resistance in plum.
  JOURNAL   PLoS One. 2013;8(1):e50627. doi: 10.1371/journal.pone.0050627. Epub 
            2013 Jan 28.
  PUBMED    23382802
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013"; volume: "8(1):e50627. doi"; pages: " 
            10.1371/journal.pone.0050627. Epub 2013 Jan 28"
COMMENT     Sequence Label: pPd4E-ISH
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    148..172
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(476..500)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(511..557)
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     primer_bind     complement(546..566)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 aacaatatat ttataaatta ttttttcaca attataacaa ctatattatt ataatcatac
       61 taattaacat cacttaacta ttttatacta aaaggaaaaa agaaaataat tatttcctta
      121 ccaagctggg gtaccgaatt cctcgagacc actttgtaca agaaagctgg gtcgtaaatg
      181 ctccagaact cctcgacggt actgaaggta tagatcgggc gaatggaact cccccaatcc
      241 tcttgcttag tcttagcgga ctttgctgcg gggctatcga accagaaggt ccatgagtgc
      301 tccagagcat gagactctgg cgctattcct ttcgatggct tcgacgccga ttcttcgtcg
      361 cccacgatct ctccttcttc gggctcatcg tcctcttccc ttggtttagg gttcgtctcg
      421 gtcttggcct gatcttctga agctgaagtc ttgagtgcgt cttcgaccac catgaagcct
      481 gcttttttgt acaaacttgt ctcgagcgtg tcctctccaa atgaaatgaa cttccttata
      541 tagaggaagg gtcttgcgaa ggatagtggg attgtgcgtc atcccttacg tcagtggaga
      601 tgtcacatca atccacttgc tttgaagacg tggttggaac gtcttctttt tccacgatgc
      661 tcctcgtggg tgggggtcca tctttgggac cactgtcggc agagagatct tgaatgatag
      721 cctttccttt atcgcaatga tggcatttgt aggagccacc ttccttttct actgtccttt
      781 cgatgaagtg acagatagct gggcaatgga atccgaggag gtttcc
//
