LOCUS       sequence-114671-         674 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses PKIalpha and nuclear localized mRuby2 (linked with IRES) 
            in a Cre-dependent manner in an AAV backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 674)
  AUTHORS   Chen Y, Saulnier JL, Yellen G, Sabatini BL
  TITLE     A PKA activity sensor for quantitative analysis of endogenous GPCR 
            signaling via 2-photon FRET-FLIM imaging.
  JOURNAL   Front Pharmacol. 2014 Apr 2;5:56. doi: 10.3389/fphar.2014.00056. 
            eCollection 2014.
  PUBMED    24765076
REFERENCE   2  (bases 1 to 674)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Pharmacol."; date: "2014-04-2"; volume: "5:56. doi"; pages: " 
            10.3389/fphar.2014.00056. eCollection 2014"
COMMENT     Sequence Label: AAV-FLEX-PKIalpha-IRES-nls-mRuby2
FEATURES             Location/Qualifiers
     source          1..674
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(104..124)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    177..638
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(336..355)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     565..582
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 ctgtgcattt gaattggtgg ccgttgaccg aaccttccat gaccaccttc atacgcatat
       61 tttccttgat cagctcttcg cccttagaca ccatggggtc ttctaccttt ctcttctttt
      121 ttggaggagt agaatgttga gagtcagcag tagcctcatc atcactagac atggtattat
      181 catcgtgttt ttcaaaggaa aaccacgtcc ccgtggttcg gggggcctag acgtttttta
      241 acctcgacta aacacatgta aagcatgtgc accgaggccc cagatcagat cccatacaat
      301 ggggtacctt ctgggcatcc ttcagcccct tgttgaatac gcttgaggag agccatttga
      361 ctctttccac aactatccaa ctcacaacgt ggcactgggg ttgtgccgcc tttgcaggtg
      421 tatcttatac acgtggcttt tggccgcaga ggcacctgtc gccaggtggg gggttccgct
      481 gcctgcaaag ggtcgctaca gacgttgttt gtcttcaaga agcttccaga ggaactgctt
      541 ccttcacgac attcaacaga ccttgcattc ctttggcgag aggggaaaga cccctaggaa
      601 tgctcgtcaa gaagacaggg ccaggtttcc gggccctcac attgccaaaa gacggcaata
      661 tggtggaaaa taac
//
