LOCUS       sequence-114708-         998 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pETDuet-1 Containing FadD V4F, W5L -His.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Ford TJ, Way JC
  TITLE     Enhancement of E. coli acyl-CoA synthetase FadD activity on medium 
            chain fatty acids.
  JOURNAL   PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 
            2015.
  PUBMED    26157619
REFERENCE   2  (bases 1 to 998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PeerJ."; 
            date: "2015-06-30"; volume: "3:e1040. doi"; pages: " 
            10.7717/peerj.1040. eCollection 2015"
COMMENT     Sequence Label: PAS531 (TJF074)
FEATURES             Location/Qualifiers
     source          1..998
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     55..74
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        55..73
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    74..98
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             113..135
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ggccacgatg cgtccggcgc tagaggatcg agatcgatct cgatcccgcg aaattaatac
       61 gactcactat aggggaattg tgagcggata acaattcccc tctagaaata attttgttta
      121 actttaagaa ggagatatac catggcattg aagaagtttc ttaaccgtta tcccgcggac
      181 gttccgacgg agatcaaccc tgaccgttat caatctctgg tagatatgtt tgagcagtcg
      241 gtcgcgcgct acgccgatca acctgcgttt gtgaatatgg gggaggtaat gaccttccgc
      301 aagctggaag aacgcagtcg cgcgtttgcc gcttatttgc aacaagggtt ggggctgaag
      361 aaaggcgatc gcgttgcgtt gatgatgcct aatttattgc aatatccggt ggcgctgttt
      421 ggcattttgc gtgccgggat gatcgtcgta aacgttaacc cgttgtatac cccgcgtgag
      481 cttgagcatc agcttaacga tagcggcgca tcggcgattg ttatcgtgtc taactttgct
      541 cacacactgg aaaaagtggt tgataaaacc gccgttcagc acgtaattct gacccgtatg
      601 ggcgatcagc tatctacggc aaaaggcacg gtagtcaatt tcgttgttaa atacatcaag
      661 cgtttggtgc cgaaatacca tctgccagat gccatttcat ttcgtagcgc actgcataac
      721 ggctaccgga tgcagtacgt caaacccgaa ctggtgccgg aagatttagc ttttctgcaa
      781 tacaccggcg gcaccactgg tgtggcgaaa ggcgcgatgc tgactcaccg caatatgctg
      841 gcgaacctgg aacaggttaa cgcgacctat ggtccgctgt tgcatccggg caaagagctg
      901 gtggtgacgg cgctgccgct gtatcacatt tttgccctga ccattaactg cctgctgttt
      961 atcgaactgg gtgggcagaa cctgcttatc actaaccc
//
