LOCUS       sequence-114711-         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  In vivo visualization of the mitochondria (can be used for 
            colocalization studies).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Goedhart J, von Stetten D, Noirclerc-Savoye M, Lelimousin M, Joosen 
            L, Hink MA, van Weeren L, Gadella TW Jr, Royant A
  TITLE     Structure-guided evolution of cyan fluorescent proteins towards a 
            quantum yield of 93%.
  JOURNAL   Nat Commun. 2012 Mar 20;3:751. doi: 10.1038/ncomms1738.
  PUBMED    22434194
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2012-03-20"; volume: "3:751. doi"; pages: " 
            10.1038/ncomms1738"
COMMENT     Sequence Label: pmTurquoise2-Mito
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    7..72
                     /label=MCS
                     /note="multiple cloning site"
     polyA_signal    196..317
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(233..252)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     287..306
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(324..779)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(461..482)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     673..692
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tgtcagtccg gactcagatc tcgagctcaa gcttcgaatt ctgcagtcga cggtaccgcg
       61 ggcccgggat ccaccggatc tagataactg atcataatca gccataccac atttgtagag
      121 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
      181 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
      241 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
      301 ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt
      361 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
      421 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
      481 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
      541 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
      601 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
      661 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
      721 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
      781 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
      841 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
//
