LOCUS       sequence-114751-        1104 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pETDuet-1 Containing FadD F447D S379R.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Ford TJ, Way JC
  TITLE     Enhancement of E. coli acyl-CoA synthetase FadD activity on medium 
            chain fatty acids.
  JOURNAL   PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 
            2015.
  PUBMED    26157619
REFERENCE   2  (bases 1 to 1104)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PeerJ."; 
            date: "2015-06-30"; volume: "3:e1040. doi"; pages: " 
            10.7717/peerj.1040. eCollection 2015"
COMMENT     Sequence Label: PAS517 (TJF084)
FEATURES             Location/Qualifiers
     source          1..1104
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    149..173
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(173..192)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(174..192)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(281..298)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tccatgtgct ggcgttcaat ttcgcagcag cggtttcttt accagactcg agggtaccga
       61 cgtcagcgat cgcgtggccg gccgatatcc aattgagatc tgccatatgt atatctcctt
      121 cttatactta actaatatac taagatgggg aattgttatc cgctcacaat tcccctatag
      181 tgagtcgtat taatttcgat tatgcggccg tgtacaatac gattactttc tgttcgactt
      241 aagcattatg cggccgcaag cttggatcct cattcattca gtgatgatga tggtgatgag
      301 aagaacctca ggctttattg tccactttgc cgcgcgcttc gtcacgtaat tctcgtcgca
      361 aaatttttcc gacgttagat ttcggtaact catcacgaaa ctccaccagc ttcggtactt
      421 tgtagcccgt gagctgacgg cggcaaaagg tcaccagtga ctcttcggta agcgatggat
      481 cttttttcac tacgaagatt ttcaccgctt caccactgga gccggaaggt acgccaacag
      541 ccgcgacttc ctgtacgcca ggatgctgca tgacgacatc ttcaatctcg ttgggataga
      601 cgttaaaacc ggaaaccaga atcatgtctt ttttacgatc gacaatgcgc agatctcctt
      661 cttcatccat caccgcgatg tcgccggtgt gtaaccagcc atttttgatg atctcatctg
      721 tagcatccgg acgctgccag taacccagca tcacctgcgg tcctttgaca caaagctcac
      781 ccggttgacc cggtggtact tcattatcat catcatccac cagtttggct tccgtcgacg
      841 gcaccggcaa accgatacgt ccactatgat aatcaatatc atatgggtta acgctgacca
      901 gcggcgcaca ctcggtaagg ccatagcctt ccagcagata ctgtcctgtc agtttcaccc
      961 aacgctctgc caccacttgc tgcactggca tccctccgcc tgcggaaaga tgcagactgg
     1021 agaaatccag ctgctggaac tctttattgt tcagcaacgc attgaacagg gtgttaacgc
     1081 cggtgatagc ggtaaacgga tatt
//
