LOCUS       sequence-114762-         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FLAG-HA expression vector with RBPMS carrying mutation K36E, R38A, 
            E39A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Teplova M, Farazi TA, Tuschl T, Patel DJ
  TITLE     Structural basis underlying CAC RNA recognition by the RRM domain of
            dimeric RNA-binding protein RBPMS.
  JOURNAL   Q Rev Biophys. 2015 Sep 8:1-11.
  PUBMED    26347403
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Q Rev 
            Biophys. 2015 Sep 8:1-11."
COMMENT     Sequence Label: pFRT-TODestFLAGHA_RBPMS_K36E_R38A_E39A
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    15..33
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     43..67
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             167..190
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             191..217
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    233..257
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cagtgataga gatctcccta tcagtgatag agatcgtcga cgagctcgtt tagtgaaccg
       61 tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg
      121 atccagcctc cggactctag cgtttaaact taagcttggt accatggact acaaggacga
      181 cgatgacaag tacccttatg acgtgcccga ttacgctttg aaccagatat caacaagttt
      241 gtacaaaaaa gcaggctcca ccatgggaac caattcagtc gacatgaaca acggcggcaa
      301 agccgagaag gagaacaccc cgagcgaggc caaccttcag gaggaggagg tccggaccct
      361 atttgtcagt ggccttcctc tggacattga acctgcggcg ctctatctgc ttttcagacc
      421 atttaagggc tatgagggtt ctcttataaa gctcacatct aaacagcctg taggttttgt
      481 cagttttgac agtcgctcag aagcagaggc tgcaaagaat gctttgaatg gcatccgctt
      541 cgatcctgaa attccgcaaa cactacgact agagtttgct aaggcaaaca cgaagatggc
      601 caagaacaaa ctcgtaggga ctccaaaccc cagtactcct ctgcccaaca ctgtacctca
      661 gttcattgcc agagagccat atgagctcac agtgcctgca ctttacccca gtagccctga
      721 agtgtgggcc ccgtaccctc tgtacccagc ggagttagcg cctgctctac ctcctcctgc
      781 tttcacctat cccgcttcac tgcatgccca gatgcgctgg ctccc
//
