LOCUS       sequence-114796-        1052 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pETDuet-1 Containing FadD Q338R.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Ford TJ, Way JC
  TITLE     Enhancement of E. coli acyl-CoA synthetase FadD activity on medium 
            chain fatty acids.
  JOURNAL   PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 
            2015.
  PUBMED    26157619
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PeerJ."; 
            date: "2015-06-30"; volume: "3:e1040. doi"; pages: " 
            10.7717/peerj.1040. eCollection 2015"
COMMENT     Sequence Label: PAS501 (TJF040)
FEATURES             Location/Qualifiers
     source          1..1052
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(4..48)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     protein_bind    157..181
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(181..200)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(182..200)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(289..306)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 agacgagtcc atgtgctggc gttcaaattt cgcagcagcg gtttctttac cagactcgag
       61 ggtaccgacg tcagcgatcg cgtggccggc cgatatccaa ttgagatctg ccatatgtat
      121 atctccttct tatacttaac taatatacta agatggggaa ttgttatccg ctcacaattc
      181 ccctatagtg agtcgtatta atttcgatta tgcggccgtg tacaatacga ttactttctg
      241 ttcgacttaa gcattatgcg gccgcaagct tggatcctca ttcattcagt gatgatgatg
      301 gtgatgagaa gaacctcagg ctttattgtc cactttgccg cgcgcttcgt cacgtaattc
      361 tcgtcgcaaa atttttccga cgttagattt cggtaactca tcacgaaact ccaccagctt
      421 cggtactttg tatcccgtga gctgacggcg gcaaaaagtc accagtgact cttcggtaag
      481 cgatggatct tttttcacta cgaagatttt caccgcttca ccactggagc cggaaggtac
      541 gccaacagcc gcgacttcct gtacgccagg atgctgcatg acgacatctt caatctcgtt
      601 gggatagacg ttaaaaccgg aaaccagaat catgtctttt ttacgatcga caatgcgcag
      661 gaatccttct tcatccatta ccgcgatgtc gccggtgtgt aaccagccat ttttgatgat
      721 ttcatcggta gcatcgggac gctgccagta acccagcatc acctgcggtc ctttgacaca
      781 aagctcaccc ggttgacctg gtggtacttc attatcatca tcatccacca gtttggcttc
      841 cgtcgacggc accggcaaac cgatgctacc actatgataa tcaatatcat atgggttaac
      901 gctgaccagc ggcgcacact cggtaaggcc atagccttcc agcagatact gtccggtcag
      961 tttcacccaa cgctctgcca ccacttgccg cactggcatt ccaccgcctg cggaaagatg
     1021 cagactggag aaatccagct gctggaactc tt
//
