LOCUS       sequence-11480-e        5294 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5294)
  AUTHORS   Allen GC, Hall G, Michalowski S, Newman W, Spiker S, Weissinger AK, 
            Thompson WF
  TITLE     High-level transgene expression in plant cells: effects of a strong 
            scaffold attachment region from tobacco.
  JOURNAL   Plant Cell. 1996 May . 8(5):899-913.
  PUBMED    8672887
REFERENCE   2  (bases 1 to 5294)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            1996 May . 8(5):899-913."
COMMENT     Sequence Label: pGHNC5
FEATURES             Location/Qualifiers
     source          1..5294
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(129..197,198..989)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(347..366)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1160..1748
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1649..1668
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1902..1919
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2072..2077,2078..2095,2096..2102)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2115..2137
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2134..2150
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2169..2189
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     3386..3402
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     3387..3403
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4650..4669)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4651..4669)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4679..4696)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4679..4695)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4688..4710)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4837..5292
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4924..4943)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5134..5155
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct gggtaccggg ccctcgatta
     2221 aaaatcccaa ttatatttgg tctaatttag tttggtattg agtaaaacaa attcgaacca
     2281 aaccaaaata taaatatata gtttttatat atatgccttt aagacttttt atagaatttt
     2341 ctttaaaaaa tatctagaaa tatttgcgac tcttctggca tgtaatattt cgttaaatat
     2401 gaagtgctcc atttttatta actttaaata attggttgta cgatcacttt cttatcaagt
     2461 gttactaaaa tgcgtcaatc tctttgttct tccatattca tatgtcaaaa tctatcaaaa
     2521 ttcttatata tctttttcga atttgaagtg aaatttcgat aatttaaaat taaatagaac
     2581 atatcattat ttaggtatca tattgatttt tatacttaat tactaaattt ggttaacttt
     2641 gaaagtgtac atcaacgaaa aattagtcaa acgactaaaa taaataaata tcatgtgtta
     2701 ttaagaaaat tctcctataa gaatatttta atagatcata tgtttgtaaa aaaaattaat
     2761 ttttactaac acatatattt acttatcaaa aatttgacaa agtaagatta aaataatatt
     2821 catctaacaa aaaaaaaacc agaaaatgct gaaaacccgg caaaaccgaa ccaatccaaa
     2881 ccgatatagt tggtttggtt tgattttgat ataaaccgaa ccaactcggt ccatttgcac
     2941 ccctaatcat aatagcttta atatttcaag atattattaa gttaacgttg tcaatatcct
     3001 ggaaattttg caaaatgaat caagcctata tggctgtaat atgaatttaa aagcagctcg
     3061 atgtggtggt aatatgtaat ttacttgatt ctaaaaaaat atcccaagta ttaataattt
     3121 ctgctaggaa gaaggttagc tacgatttac agcaaagcca gaatacaaag aaccataaag
     3181 tgattgaagc tcgaaatata cgaaggaaca aatattttta aaaaaatacg caatgacttg
     3241 gaacaaaaga aagtgatata ttttttgttc ttaaacaagc atcccctcta aagaatggca
     3301 gttttccttt gcatgtaact attatgctcc cttcgttaca aaaattttgg actactattg
     3361 ggaacttctt ctgaaaatag tcccctcgag gtcgacggta tcgataagct tgatatcgaa
     3421 ttcctgcagc ccgggggatc cactagttcg attaaaaatc ccaattatat ttggtctaat
     3481 ttagtttggt attgagtaaa acaaattcga accaaaccaa aatataaata tatagttttt
     3541 atatatatgc ctttaagact ttttatagaa ttttctttaa aaaatatcta gaaatatttg
     3601 cgactcttct ggcatgtaat atttcgttaa atatgaagtg ctccattttt attaacttta
     3661 aataattggt tgtacgatca ctttcttatc aagtgttact aaaatgcgtc aatctctttg
     3721 ttcttccata ttcatatgtc aaaatctatc aaaattctta tatatctttt tcgaatttga
     3781 agtgaaattt cgataattta aaattaaata gaacatatca ttatttaggt atcatattga
     3841 tttttatact taattactaa atttggttaa ctttgaaagt gtacatcaac gaaaaattag
     3901 tcaaacgact aaaataaata aatatcatgt gttattaaga aaattctcct ataagaatat
     3961 tttaatagat catatgtttg taaaaaaaat taatttttac taacacatat atttacttat
     4021 caaaaatttg acaaagtaag attaaaataa tattcatcta acaaaaaaaa aaccagaaaa
     4081 tgctgaaaac ccggcaaaac cgaaccaatc caaaccgata tagttggttt ggtttgattt
     4141 tgatataaac cgaaccaact cggtccattt gcacccctaa tcataatagc tttaatattt
     4201 caagatatta ttaagttaac gttgtcaata tcctggaaat tttgcaaaat gaatcaagcc
     4261 tatatggctg taatatgaat ttaaaagcag ctcgatgtgg tggtaatatg taatttactt
     4321 gattctaaaa aaatatccca agtattaata atttctgcta ggaagaaggt tagctacgat
     4381 ttacagcaaa gccagaatac aaagaaccat aaagtgattg aagctcgaaa tatacgaagg
     4441 aacaaatatt tttaaaaaaa tacgcaatga cttggaacaa aagaaagtga tatatttttt
     4501 gttcttaaac aagcatcccc tctaaagaat ggcagttttc ctttgcatgt aactattatg
     4561 ctcccttcgt tacaaaaatt ttggactact attgggaact tcttctgaaa atagtagagc
     4621 ggccgccacc gcggtggagc tccaattcgc cctatagtga gtcgtattac gcgcgctcac
     4681 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     4741 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     4801 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa
     4861 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     4921 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     4981 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     5041 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     5101 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     5161 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     5221 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     5281 cgcttacaat ttag
//
