LOCUS       sequence-114872-        1060 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pETDuet-1 Containing FadD R449G.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Ford TJ, Way JC
  TITLE     Enhancement of E. coli acyl-CoA synthetase FadD activity on medium 
            chain fatty acids.
  JOURNAL   PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 
            2015.
  PUBMED    26157619
REFERENCE   2  (bases 1 to 1060)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PeerJ."; 
            date: "2015-06-30"; volume: "3:e1040. doi"; pages: " 
            10.7717/peerj.1040. eCollection 2015"
COMMENT     Sequence Label: PAS521 (TJF088)
FEATURES             Location/Qualifiers
     source          1..1060
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    73..97
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             112..134
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ggccacgatg cgtccggcgt agaggatcga gatcgatctc gatcccgcga aattaatacg
       61 actcactata ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa
      121 ctttaagaag gagatatacc atggcattga agaaggtttg gcttaaccgt tatcccgcgg
      181 acgttccgac ggagatcaac cctgaccgtt atcaatctct ggtagatatg tttgagcagt
      241 cggtcgcgcg ctacgccgat caacctgcgt ttgtgaatat gggggaggta atgaccttcc
      301 gcaagctgga agaacgcagt cgcgcgtttg ccgcttattt gcaacaaggg ttggggctga
      361 agaaaggcga tcgcgttgcg ttgatgatgc ctaatttatt gcaatatccg gtggcgctgt
      421 ttggcatttt gcgtgccggg atgatcgtcg taaacgttaa cccgttgtat accccgcgtg
      481 agcttgagca tcagcttaac gatagcggcg catcggcgat tgttatcgtg tctaactttg
      541 ctcacacact ggaaaaagtg gttgataaaa ccgccgttca gcacgtaatt ctgacccgta
      601 tgggcgatca gctatctacg gcaaaaggca cggtagtcaa tttcgttgtt aaatacatca
      661 agcgtttggt gccgaaatac catctgccag atgccatttc atttcgtagc gcactgcata
      721 acggctaccg gatgcagtac gtcaaacccg aactggtgcc ggaagattta gcttttctgc
      781 aatacaccgg cggcaccact ggtgtggcga aaggcgcgat gctgactcac cgcaatatgc
      841 tggcgaacct ggaacaggtt aacgcgacct atggtccgct gttgcatccg ggcaaagagc
      901 tggtggtgac ggcgctgccg ctgtatcaca tttttgccct gaccattaac tgcctgctgt
      961 ttatcgaact gggtgggcag aacctgctta tcactaaccc gcgcgatatt ccagggttgg
     1021 taaaagagtt agcgaaatat ccgtttaccg ctatcccggg
//
