LOCUS       sequence-114877-         939 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  baculovirus-based delivery system that enables the efficient 
            incorporation of unnatural amino acids into proteins in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Chatterjee A, Xiao H, Bollong M, Ai HW, Schultz PG
  TITLE     Efficient viral delivery system for unnatural amino acid mutagenesis
            in mammalian cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Jul 16;110(29):11803-8. doi: 
            10.1073/pnas.1309584110. Epub 2013 Jul 1.
  PUBMED    23818609
REFERENCE   2  (bases 1 to 939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1309584110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-07-16- 16"; volume: "110"; issue: "29"; pages: "11803-8"
COMMENT     Sequence Label: pAcBac2.tR4-OMeYRS/GFP*
FEATURES             Location/Qualifiers
     source          1..939
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(163..257)
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     protein_bind    163..181
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    191..209
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(227..246)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     promoter        complement(347..595)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(405..424)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(575..595)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     polyA_signal    632..856
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     845..862
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 ggctatggca gggcttgcgc ttaatgcgcc gctacagggc gcgtggggat accccctaga
       61 gccccagctg aaaaaatgga gggggacgga ttcgaaccgc cgaacccaaa gggagcggat
      121 ttagagtccg ccgcgtttag ccacttcgct acccctccgg tgtctctatc actgataggg
      181 aacttataag tctctatcac tgatagggat ttcacgttta tggtgatttc ccagaacaca
      241 tagcgacatg caaatattaa aaaatggtgg gggaaggatt cgaaccttcg aagtctgtga
      301 cggcagattt agagtctgct ccctttggcc gctcgggaac cccaccggtg tttcgtcctt
      361 tccacaagat atataaagcc aagaaatcga aatactttca agttacggta agcatatgat
      421 agtccatttt aaaacataat tttaaaactg caaactaccc aagaaattat tactttctac
      481 gtcacgtatt ttgtactaat atctttgtgt ttacagtcaa attaattcta attatctctc
      541 taacagcctt gtatcgtata tgcaaatatg aaggaatcat gggaaatagg ccctcttcct
      601 gcccgagatc tggttctttc cgcctcagaa gccatagagc ccaccgcatc cccagcatgc
      661 ctgctattgt cttcccaatc ctcccccttg ctgtcctgcc ccaccccacc ccccagaata
      721 gaatgacacc tactcagaca atgcgatgca atttcctcat tttattagga aaggacagtg
      781 ggagtggcac cttccagggt caaggaaggc acgggggagg ggcaaacaac agatggctgg
      841 caactagaag gcacagtcga ggctgatcag cgggtttaaa cgggccctct agactcgagt
      901 taaagtcgac ttaacgcgtt gaattcttaa acgggccct
//
