LOCUS       sequence-114897-         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pETDuet-1 Containing FadD V4F, W5L.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Ford TJ, Way JC
  TITLE     Enhancement of E. coli acyl-CoA synthetase FadD activity on medium 
            chain fatty acids.
  JOURNAL   PeerJ. 2015 Jun 30;3:e1040. doi: 10.7717/peerj.1040. eCollection 
            2015.
  PUBMED    26157619
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PeerJ."; 
            date: "2015-06-30"; volume: "3:e1040. doi"; pages: " 
            10.7717/peerj.1040. eCollection 2015"
COMMENT     Sequence Label: PAS503 (TJF066)
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     57..76
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        57..75
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    76..100
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             115..137
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ccggccacga atgcgtccgg cgtagaggat cgagatcgat ctcgatcccg cgaaattaat
       61 acgactcact ataggggaat tgtgagcgga taacaattcc cctctagaaa taattttgtt
      121 taactttaag aaggagatat accatggcat tgaagaagtt tcttaaccgt tatcccgcgg
      181 acgttccgac ggagatcaac cctgaccgtt atcaatctct ggtagatatg tttgagcagt
      241 cggtcgcgcg ctacgccgat caacctgcgt ttgtgaatat gggggaggta atgaccttcc
      301 gcaagctgga agaacgcagt cgcgcgtttg ccgcttattt gcaacaaggg ttggggctga
      361 agaaaggcga tcgcgttgcg ttgatgatgc ctaatttatt gcaatatccg gtggcgctgt
      421 ttggcatttt gcgtgccggg atgatcgtcg taaacgttaa cccgttgtat accccgcgtg
      481 agcttgagca tcagcttaac gatagcggcg catcggcgat tgttatcgtg tctaactttg
      541 ctcacacact ggaaaaagtg gttgataaaa ccgccgttca gcacgtaatt ctgacccgta
      601 tgggcgatca gctatctacg gcaaaaggca cggtagtcaa tttcgttgtt aaatacatca
      661 agcgtttggt gccgaaatac catctgccag atgccatttc atttcgtagc gcactgcata
      721 acggctaccg gatgcagtac gtcaaacccg aactggtgcc ggaagattta gcttttctgc
      781 aatacaccgg cggcaccact ggtgtggcga aaggcgcgat gctgactcac cgcaatatgc
      841 tggcgaacct ggaacaggtt aacgc
//
