LOCUS       sequence-114959-         730 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FLAG-HA expression vector with RBPMS carrying mutation K100A and 
            encoding silent mutation to render resistant to siRNA R2 (Farazi et 
            al. 2014).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Teplova M, Farazi TA, Tuschl T, Patel DJ
  TITLE     Structural basis underlying CAC RNA recognition by the RRM domain of
            dimeric RNA-binding protein RBPMS.
  JOURNAL   Q Rev Biophys. 2015 Sep 8:1-11.
  PUBMED    26347403
REFERENCE   2  (bases 1 to 730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Q Rev 
            Biophys. 2015 Sep 8:1-11."
COMMENT     Sequence Label: pFRT-TODestFLAGHA_RBPMS_KDR_K100A
FEATURES             Location/Qualifiers
     source          1..730
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(33..74)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    127..151
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tcagttatct atgcggccgc ggatcaggta cgcgtagaat cgagaccgag gagagggtta
       61 gggataggct taccttcgaa ccgcgggccc tctagacttg agcggccgcc actgtgctgg
      121 atatcaacca ctttgtacaa gaaagctggg tctagatatc tcgagtgcgg ccgctcagca
      181 gaactgacgg gacttccagc cctgagaagt agcctcggag ggagggagcc agcgcatctg
      241 ggcatgcagt gaagcgggat aggtgaaagc aggaggaggt agagcaggcg ctaactccgc
      301 tgggtacaga gggtacgggg cccacacttc agggctactg gggtaaagtg caggcactgt
      361 gagctcatat ggctctctgg caatgaactg aggtacagtg ttgggcagag gagtactggg
      421 gtttggagtc cctacgagtt tgttcttggc catcttcgtg tttgccgcag caaactctag
      481 tcgtagtgtt tgcggaattt caggatcgaa gcggatgcca ttcaaagcat tctttgcagc
      541 ctctgcttct gagcgactgt caaaactgac aaaacctaca ggctgtttag atgtgagctt
      601 tataagagaa ccctcatagc ccttaaatgg tctgaaaagc agatagagct cccgaggttt
      661 aatgtccaga ggaaggccac tgacaaatag ggtccggacc tcctcctcct gaaggttggc
      721 ctcgctcggg
//
