LOCUS       sequence-114996-         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses FLAGHA-tagged HuD in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  TITLE     Identification of the transcriptome-wide mRNA targets of the 
            neuronal ELAVL proteins (unpublished)
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFRT-TODestFLAGHA_HuD
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    21..39
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     49..73
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             173..196
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             197..223
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    239..263
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ccctatcagt gatagagatc tccctatcag tgatagagat cgtcgacgag ctcgtttagt
       61 gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg
      121 ggaccgatcc agcctccgga ctctagcgtt taaacttaag cttggtacca tggactacaa
      181 ggacgacgat gacaagtacc cttatgacgt gcccgattac gctttgaacc agatatcaac
      241 aagtttgtac aaaaaagcag gctccaccat gggaaccaat tcagtcgaca tggttatgat
      301 aattagcacc atggagcctc aggtgtcaaa tggtccgaca tccaatacaa gcaatggacc
      361 ctccagcaac aacagaaact gtccttctcc catgcaaaca ggggcaacca cagatgacag
      421 caaaaccaac ctcatcgtca actatttacc ccagaatatg acccaagaag aattcaggag
      481 tctcttcggg agcattggtg aaatagaatc ctgcaaactt gtgagagaca aaattacagg
      541 acagagttta gggtatggat ttgttaacta tattgatcca aaggatgcag agaaagccat
      601 caacacttta aatggactca gactccagac caaaaccata aaggtctcat atgcccgtcc
      661 gagctctgcc tcaatcaggg atgctaacct ctatgttagc ggccttccca aaaccatgac
      721 ccagaaggaa ctggagcaac ttttctcgca atacggccgt atcatcacct cacgaatcct
      781 ggttgatcaa gtcacaggag tgtccagagg ggtgggattc atccgctttg at
//
