LOCUS       sequence-115-exp        4574 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4574)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 4574)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD96_32
FEATURES             Location/Qualifiers
     source          1..4574
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(251..279)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1027..1047
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(2014..2036)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2136..2155
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2233..2251)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2319..2423
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2424..2492,2493..3284)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2642..2661)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3455..4043
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3944..3963
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4197..4214
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4331..4352
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4367..4372,4373..4390,4391..4397)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4405..4421
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4410..4432
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4429..4445
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4429..4445
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ggatccccgg gattggccaa aggacccaaa
       61 ggtatgtttc gaatgatact aacataacat agaacatttt caggaggacc cttggagggt
      121 acctcgagaa aaaatggcac tcctgcaatc acgtctcctg ctatccgcac ctcgtcgtgc
      181 cgctgcaact gcaagagctt catccgtacc ggtagaaaaa atgagtaaag gagaagaact
      241 tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt
      301 ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat
      361 ttgcactact ggaaaactac ctgttccatg ggtaagttta aacatatata tactaactaa
      421 ccctgattat ttaaattttc agccaacact tgtcactact ttctgttatg gtgttcaatg
      481 cttctcgaga tacccagatc atatgaaacg gcatgacttt ttcaagagtg ccatgcccga
      541 aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtaa
      601 gtttaaacag ttcggtacta actaaccata catatttaaa ttttcaggtg ctgaagtcaa
      661 gtttgaaggt gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga
      721 tggaaacatt cttggacaca aattggaata caactataac tcacacaatg tatacatcat
      781 ggcagacaaa caaaagaatg gaatcaaagt tgtaagttta aacatgattt tactaactaa
      841 ctaatctgat ttaaattttc agaacttcaa aattagacac aacattgaag atggaagcgt
      901 tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc
      961 agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga
     1021 ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact
     1081 atacaaatag cattcgtaga attccaactg agcgccggtc gctaccatta ccaacttgtc
     1141 tggtgtcaaa aataataggg gccgctgtca tcagagtaag tttaaactga gttctactaa
     1201 ctaacgagta atatttaaat tttcagcatc tcgcgcccgt gcctctgact tctaagtcca
     1261 attactcttc aacatcccta catgctcttt ctccctgtgc tcccaccccc tatttttgtt
     1321 attatcaaaa aacttctctt aatttctttg ttttttagct tcttttaagt cacctctaac
     1381 aatgaaattg tgtagattca aaaatagaat taattcgtaa taaaaagtcg aaaaaaattg
     1441 tgctccctcc ccccattaat aataattcta tcccaaaatc tacacaatgt tctgtgtaca
     1501 cttcttatgt tttttacttc tgataaattt ttttgaaaca tcatagaaaa aaccgcacac
     1561 aaaatacctt atcatatgtt acgtttcagt ttatgaccgc aatttttatt tcttcgcacg
     1621 tctgggcctc tcatgacgtc aaatcatgct catcgtgaaa aagttttgga gtatttttgg
     1681 aatttttcaa tcaagtgaaa gtttatgaaa ttaattttcc tgcttttgct ttttggggtt
     1741 tcccctattg tttgtcaaga tttcgaggac ggcgtttttc ttgctaaaat cacaagtatt
     1801 gatgagcacg atgcaagaaa gatcggaaga aggtttgggt ttgaggctca gtggaaggtg
     1861 agtagaagtt gataatttga aagtggagta gtgtctatgg ggtttttgcc ttaaatgaca
     1921 gaatacattc ccaatatacc aaacataact gtttcctact agtcggccgt acgggccctt
     1981 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     2041 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     2101 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag
     2161 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
     2221 gcggccttaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     2281 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     2341 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     2401 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     2461 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     2521 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     2581 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     2641 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     2701 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     2761 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     2821 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     2881 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     2941 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     3001 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     3061 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     3121 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     3181 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     3241 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     3301 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     3361 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     3421 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     3481 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     3541 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     3601 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     3661 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     3721 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     3781 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     3841 gtgagcattg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     3901 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     3961 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     4021 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     4081 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     4141 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     4201 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     4261 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     4321 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     4381 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     4441 atgaccatga ttacgccaag ctgtaagttt aaacatgatc ttactaacta actattctca
     4501 tttaaatttt cagagcttaa aaatggctga aatcactcac aacgatggat acgctaacaa
     4561 cttggaaatg aaat
//
