LOCUS       sequence-115000-         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  constitutively expresses FLAG-HA-tagged HuC in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  TITLE     Identification of the transcriptome-wide mRNA targets of the 
            neuronal ELAVL proteins (unpublished)
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFRT-DestFLAGHA_HuC
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     31..50
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        31..49
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      114..123
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             123..146
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             147..173
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    189..213
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agaacccact gcttactggc ttatcgaaat taatacgact cactataggg agacccaagc
       61 tggctagcga gctcggatcg atccatcgat agtgtggtgg aattctgatc gccgccgcca
      121 tggactacaa ggacgacgat gacaagtacc cttatgacgt gcccgattac gctttgaacc
      181 agatatcaac aagtttgtac aaaaaagcag gctccaccat gggaaccaat tcagtcgaca
      241 tggtcactca gatactgggg gccatggagt ctcaggtggg ggggggcccg gccggcccgg
      301 ccctgcccaa cgggccactc cttggtacaa atggagccac tgacgacagc aagaccaacc
      361 tcatcgtcaa ctacctgccc cagaacatga cccaggatga gttcaagagt ctcttcggca
      421 gcattggcga catcgagtcc tgcaagttgg ttcgggacaa gatcacaggg cagagccttg
      481 gctacgggtt tgtgaactat tctgacccca atgatgcaga caaagccatc aacaccctca
      541 acggcctcaa attacagacg aagaccatca aggtgtccta tgccagaccc agttcagcat
      601 ccatccggga tgctaacctg tacgtcagcg ggctccccaa gaccatgagc cagaaagaga
      661 tggagcagct cttctcccag tacggccgca tcatcacgtc ccgcatcctg gtggaccagg
      721 tcacaggtgt ctctcggggt gtgggattca tccgctttga caagaggatt gaggccgaag
      781 aggctatcaa aggactgaat gggcagaagc cgctgggcgc
//
