LOCUS       sequence-115017-         463 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pSC101 based plasmid where pl-tetO expression drives synthesis of 
            construct expressing (N to C terminal) MBP (Maltose Binding 
            Protein), Ntag, and mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Sekar K, Gentile AM, Bostick JW, Tyo KE
  TITLE     N-Terminal-Based Targeted, Inducible Protein Degradation in 
            Escherichia coli.
  JOURNAL   PLoS One. 2016 Feb 22;11(2):e0149746. doi: 
            10.1371/journal.pone.0149746. eCollection 2016.
  PUBMED    26900850
REFERENCE   2  (bases 1 to 463)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-02-22"; volume: "11(2):e0149746. doi"; pages: " 
            10.1371/journal.pone.0149746. eCollection 2016"
COMMENT     Sequence Label: pKS011
FEATURES             Location/Qualifiers
     source          1..463
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atcagcagga cgcactgaca ggtaccgcat gaaaataaaa acaggtgcac gcatcctcgc
       61 attatccgca ttaacgacga tgatgttttc cgcctcggct ctcgccaaaa tcgaagaagg
      121 taaactggta atctggatta acggcgataa aggctataac ggtctcgctg aagtcggtaa
      181 gaaattcgag aaagataccg gaattaaagt caccgttgag catccggata aactggaaga
      241 gaaattccca caggttgcgg caactggcga tggccctgac attatcttct gggcacacga
      301 ccgctttggt ggctacgctc aatctggcct gttggctgaa atcaccccgg acaaagcgtt
      361 ccaggacaag ctgtatccgt ttacctggga tgccgtacgt tacaacggca agctgattgc
      421 ttacccgatc gctgttgaag cgttatcgct gatttataac aaa
//
