LOCUS       sequence-115053-         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination vector with RBPMS resistant to siRNA treatment by s21729
            (Applied Biosystems) with F65A mutation for stable cell line 
            generation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Teplova M, Farazi TA, Tuschl T, Patel DJ
  TITLE     Structural basis underlying CAC RNA recognition by the RRM domain of
            dimeric RNA-binding protein RBPMS.
  JOURNAL   Q Rev Biophys. 2015 Sep 8:1-11.
  PUBMED    26347403
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Q Rev 
            Biophys. 2015 Sep 8:1-11."
COMMENT     Sequence Label: pFRT-TODestFLAGHA_RBPMS_KDR_F65A
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(28..69)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    122..146
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tatctatgcg gccgcggatc aggtacgcgt agaatcgaga ccgaggagag ggttagggat
       61 aggcttacct tcgaaccgcg ggccctctag acttgagcgg ccgccactgt gctggatatc
      121 aaccactttg tacaagaaag ctgggtctag atatctcgag tgcggccgct cagcagaact
      181 gacgggactt ccagccctga gaagtagcct cggagggagg gagccagcgc atctgggcat
      241 gcagtgaagc gggataggtg aaagcaggag gaggtagagc aggcgctaac tccgctgggt
      301 acagagggta cggggcccac acttcagggc tactggggta aagtgcaggc actgtgagct
      361 catatggctc tctggcaatg aactgaggta cagtgttggg cagaggagta ctggggtttg
      421 gagtccctac gagtttgttc ttggccatct tcgtgtttgc cttagcaaac tctagtcgta
      481 gtgtttgcgg aatttcagga tcgaagcgga tgccattcaa agcattcttt gcagcctctg
      541 cttctgagcg actgtcaaaa ctgacagcac ctacaggctg tttagatgtg agctttataa
      601 gagaaccctc atagccctta aatggtctga aaagcagata gagctcccga ggtttaatgt
      661 ccagaggaag gccactgaca aatagggtcc ggacctcctc ctcctgaagg ttggcctcgc
      721 tcggggtgtt ctccttctcg gctttgccgc cgttgttcat gtcgactgaa ttggt
//
