LOCUS       sequence-11506-e         495 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Karanicolas J, Corn JE, Chen I, Joachimiak LA, Dym O, Peck SH, 
            Albeck S, Unger T, Hu W, Liu G, Delbecq S, Montelione GT, Spiegel 
            CP, Liu DR, Baker D
  TITLE     A de novo protein binding pair by computational design and directed 
            evolution.
  JOURNAL   Mol Cell. 2011 Apr 22;42(2):250-60. Epub 2011 Mar 31.
  PUBMED    21458342
REFERENCE   2  (bases 1 to 495)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2011-04-22"; volume: "42(2)"; pages: "250-60. Epub 2011 Mar 
            31"
COMMENT     Sequence Label: PrbC5
FEATURES             Location/Qualifiers
     source          1..495
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             31..51
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atgaaatctt ctcaccatca ccatcaccat gaaaacctgt acttccaatc caatgcaatg
       61 ggatccaccc gtccgattga tggtctgacg gacgaaggta tccgcgagat tctgactcgt
      121 tataaaaaga tcgctctggt gggggcctct ccgaaaccgg aacgcgacgc aaatattgtt
      181 atgaaatatc tgctggagca tggctatgat gtctacccgg taaacccgaa ttatgaagaa
      241 gtgctgggcc gcaagtgcta cccgagtgtt ctggacatcc cggataaaat tgaggtcgta
      301 gatctgtttg tgaacccggc taaagcgtgg cgcttcgttg cctatgccat caagaaaggg
      361 gcaaaagtgg tatggtttca gtacaacaca tattatccgc tggctgcgcg tcaagccaag
      421 ggagcaggcc tgattatcgt tgctaatcgc tgtatgatgc gtgagcacga acgcctgctg
      481 ggcgaaaaat aataa
//
