LOCUS       sequence-115187-         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Single vector system expressing NEF allowing myristoylation of 
            recombinant protein produce in Ecoli system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Gluck JM, Hoffmann S, Koenig BW, Willbold D
  TITLE     Single vector system for efficient N-myristoylation of recombinant 
            proteins in E. coli.
  JOURNAL   PLoS One. 2010 Apr 9;5(4):e10081. doi: 10.1371/journal.pone.0010081.
  PUBMED    20404920
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2010-04-9"; volume: "5(4):e10081. doi"; pages: " 
            10.1371/journal.pone.0010081"
COMMENT     Sequence Label: pETDuet-1∆HisTag_Nef_MCS2
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             681..704
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             705..722
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 acaattcccc atcttagtat attagttaag tataagaagg agatatacat atgggtggca
       61 agtggtcaaa acgtagtatg ggtggatggt ctgctataag ggaaagaatg agacgagctg
      121 agccacgagc tgagccagca gcagatgggg tgggagcagt atctcgagac ctggaaaaac
      181 atggagcaat cacaagtagc aatacagcag ctactaatgc tgattgtgcc tggctagaag
      241 cacaagagga ggaagaggtg ggttttccag tcagacctca ggtaccttta agaccaatga
      301 cttacaaggc agctttagat attagccact ttttaaaaga aaagggggga ctggaagggc
      361 taatttggtc ccaaagaaga caagagatcc ttgatctgtg gatctaccac acacaaggct
      421 acttccctga ttggcagaat tacacaccag ggccagggat cagatatcca ctgacctttg
      481 gatggtgctt caagctagta ccagttgagc cagagaaggt agaagaggcc aatgaaggag
      541 agaacaacag cttgttacac cctatgagcc tacatgggat ggaggacgcg gagaaagaag
      601 tgttagtgtg gaggtttgac agcaaactag catttcatca catggcccga gagctgcatc
      661 cggagtacta caaagactgc ctcgaagttc tgttccaggg gccccatcac catcatcacc
      721 actaacaatt ggatatcggc cggccacgcg atcgctgacg tcggtaccct cgagtctggt
      781 aaagaaaccg ctgctgcgaa
//
