LOCUS       sequence-115219-         746 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TpR mini-Tn7 vector, mobilizable.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Choi KH, Schweizer HP
  TITLE     mini-Tn7 insertion in bacteria with single attTn7 sites: example 
            Pseudomonas aeruginosa.
  JOURNAL   Nat Protoc. 2006;1(1):153-61.
  PUBMED    17406227
REFERENCE   2  (bases 1 to 746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Protoc."; date: "2006"; volume: "1(1)"; pages: "153-61"
COMMENT     Sequence Label: pUC18T-mini-Tn7T-Tp
FEATURES             Location/Qualifiers
     source          1..746
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     294..310
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     295..311
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      339..433
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      536..622
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    673..720
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 tcttcgctat tacgccagct agaggaccag ccgcgtaacc tggcaaaatc ggttacggtt
       61 gagtaataaa tggatgccct gcgtaagcag gtgtgggcgg acaataaagt cttaaactga
      121 acaaaataga tctaaactat gacaataaag tcttaaacta gacagaatag ttgtaaactg
      181 aaatcagtcc agttatgctg tgaaaaagca tactggactt ttgttatggc taaagcaaac
      241 tcttcatttt ctgaagtgca aattgcccgt cgtattaaag aggggcgtgg ggttcgaggt
      301 cgacggtatc gataagctag cttaattagc tgagcttgga ctcctgttga tagatccagt
      361 aatgacctca gaactccatc tggatttgtt cagaacgctc ggttgccgcc gggcgttttt
      421 tattggtgag aatccaagct agactgcgat gagtggcagg gcggggcgta atttttttaa
      481 ggcagttatt ggtgccctta aacgcctggg gtaatgactc tctagcttga ggcatcaaat
      541 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
      601 cgctctcctg agtaggacaa atccgccgct aggagcttgc ggcccggacg atgagctcga
      661 attggggatc ttgaagtacc tattccgaag ttcctattct ctagaaagta taggaacttc
      721 agagcgcttt tgaagctgat gtgctt
//
