LOCUS       sequence-115277-         647 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  source of GmR FRT cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Choi KH, Gaynor JB, White KG, Lopez C, Bosio CM, Karkhoff-Schweizer 
            RR, Schweizer HP
  TITLE     A Tn7-based broad-range bacterial cloning and expression system.
  JOURNAL   Nat Methods. 2005 Jun;2(6):443-8.
  PUBMED    15908923
REFERENCE   2  (bases 1 to 647)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2005-06"; volume: "2(6)"; pages: "443-8"
COMMENT     Sequence Label: pFGM1
FEATURES             Location/Qualifiers
     source          1..647
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(55..102)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
ORIGIN
        1 tcgactctag aggatccccg ggtaccgagc tcgaattagc ttcaaaagcg ctctgaagtt
       61 cctatacttt ctagagaata ggaacttcgg aataggaact acaagatccc ctgattccct
      121 ttgtcaacag caatggatcg aattggccgc ggcgttgtga caatttaccg aacaactccg
      181 cggccgggaa gccgatctcg gcttgaacga attgttaggt ggcggtactt gggtcgatat
      241 caaagtgcat cacttcttcc cgtatgccca actttgtata gagagccact gcgggatcgt
      301 caccgtaatc tgcttgcacg tagatcacat aagcaccaag cgcgttggcc tcatgcttga
      361 ggagattgat gagcgcggtg gcaatgccct gcctccggtg ctcgccggag actgcgagat
      421 catagatata gatctcacta cgcggctgct caaacttggg cagaacgtaa gccgcgagag
      481 cgccaacaac cgcttcttgg tcgaaggcag caagcgcgat gaatgtctta ctacggagca
      541 agttcccgag gtaatcggag tccggctgat gttgggagta ggtggctacg tctccgaact
      601 cacgaccgaa aagatcaaga gcagcccgca tggatttgac ttggtca
//
