LOCUS       sequence-115286-         543 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the E105K/E108A mutant of Staphylococcus aureus sortase A 
            in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Hirakawa H, Ishikawa S, Nagamune T
  TITLE     Ca -independent sortase-A exhibits high selective protein ligation 
            activity in the cytoplasm of E. coli.
  JOURNAL   Biotechnol J. 2015 Apr 10. doi: 10.1002/biot.201500012.
  PUBMED    25864513
REFERENCE   2  (bases 1 to 543)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            J. 2015 Apr 10. doi: 10.1002/biot.201500012."
COMMENT     Sequence Label: pASKt15C+SrtA2
FEATURES             Location/Qualifiers
     source          1..543
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aaaaatgtcg cacaatgtgc gccatttttc acttcacagg tcgtttaaac ttatttgact
       61 tctgtagcta caaagatttt acgtttttcc caaacgcctg tcttttcatt gtaatcatca
      121 caagtaatta atgttaattg tttatcttta cctttttgtt catctagaac ttctacatct
      181 gtaggcttaa catctcttat acttgtcatt ttatacttac gtgtttcatt accaacttta
      241 aagtacacca tactaccttt tttggctgct ttaagatttg taaattgata gttcggacgg
      301 tcaatgaaag tgtgtcctgc aattgaaata ttttgatcat ctagtgaggc attttccttt
      361 gcaaagctta cacctctatt taattgttca ggtgttgctg gtcctggata tactggttct
      421 ttaatatcag catctggaat ttcaatatag cctgccactt tcgatttatc tttcggaatt
      481 tgaggtttag cttgggtacc cagatctggg ctgtccatgt gctggcgttc gaatttagca
      541 gcc
//
