LOCUS       sequence-115301-         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of His-tagged Med25-VBD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Sela D, Chen L, Martin-Brown S, Washburn MP, Florens L, Conaway JW, 
            Conaway RC
  TITLE     Endoplasmic reticulum stress-responsive transcription factor 
            ATF6alpha directs recruitment of the Mediator of RNA polymerase II 
            transcription and multiple histone acetyltransferase complexes.
  JOURNAL   J Biol Chem. 2012 Jun 29;287(27):23035-45. doi: 
            10.1074/jbc.M112.369504. Epub 2012 May 10.
  PUBMED    22577136
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.369504"; journalName: "J Biol Chem"; date: 
            "2012-06-29- 29"; volume: "287"; issue: "27"; pages: "23035-45"
COMMENT     Sequence Label: pET21a-Med25-VBD-6His
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(15..32)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(498..530)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     RBS             538..560
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    575..599
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(599..618)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(600..618)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(702..721)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gcagccggat ctcagtggtg gtggtggtgg tgctcgaggt tggtgatgac ctgccggatg
       61 ccgttgacga agccgctctg gtcgtagggg atgaggccca tgaagatctt cttcttggac
      121 gagtacagga gcatgagcac gcgcacctca cagggcgccg tgtgggggaa gtgcacgcag
      181 cccgcgaagc cgttgcccat gatgcggtag aggcctttga gagactccag gtccttgttg
      241 gtgaaatgga actggaccat ccttgagttc cggaacaaag ggcccagggt ggtcagcagc
      301 tgctggggga tgagctgcat gatcagcttc tggggccact gctccgtctt caggttctcg
      361 ccatgattca cgtagacctg gcagggcagt gaccgcgtca gcttggtgtt ggcatccact
      421 gaggcaggtt tgggtttctc ttgccactcc aggaccccgc tccaggccag aagcttattg
      481 gagactgagg atccgcgacc catttgctgt ccaccagtca tgctagccat atgtatatct
      541 ccttcttaaa gttaaacaaa attatttcta gaggggaatt gttatccgct cacaattccc
      601 ctatagtgag tcgtattaat ttcgcgggat cgagatctcg atcctctacg ccggacgcat
      661 cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac
      721 cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat
      781 ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccaattcc
      841 ttgcggcggc ggtgctcaac
//
