LOCUS       sequence-115327-        1158 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral introduction of Cas9 into mammalian cell line..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Shi J, Wang E, Milazzo JP, Wang Z, Kinney JB, Vakoc CR
  TITLE     Discovery of cancer drug targets by CRISPR-Cas9 screening of protein
            domains.
  JOURNAL   Nat Biotechnol. 2015 May 11. doi: 10.1038/nbt.3235.
  PUBMED    25961408
REFERENCE   2  (bases 1 to 1158)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2015 May 11. doi: 10.1038/nbt.3235."
COMMENT     Sequence Label: MSCV_Cas9_puro
FEATURES             Location/Qualifiers
     source          1..1158
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..24
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     CDS             complement(71..118)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 cagcggggct gctaaagcgc atgctccaga ctgccttggg aaaagcgcct cccctacccg
       61 gtagtcatca tttctttttc ttagcttgac cagctttctt agtagcagca ggacgcttgt
      121 cgcctcccag ctgagacagg tcgatccgtg tctcgtacag gccggtgatg ctctggtgga
      181 tcagggtggc gtccagcacc tctttggtgc tggtgtacct cttccggtcg atggtggtgt
      241 caaagtactt gaaggcggca ggggctccca gattggtcag ggtaaacagg tggatgatat
      301 tctcggcctg ctctctgatg ggcttatccc ggtgcttgtt gtaggcggac agcactttgt
      361 ccagattagc gtcggccagg atcactctct tggagaactc gctgatctgc tcgatgatct
      421 cgtccaggta gtgcttgtgc tgttccacaa acagctgttt ctgctcatta tcctcggggg
      481 agcccttcag cttctcatag tggctggcca ggtacaggaa gttcacatat ttggagggca
      541 gggccagttc gtttcccttc tgcagttcgc cggcagaggc cagcattctc ttccggccgt
      601 tttccagctc gaacagggag tacttaggca gcttgatgat caggtccttt ttcacttctt
      661 tgtagccctt ggcttccaga aagtcgatgg gattcttctc gaagctgctt ctttccatga
      721 tggtgatccc cagcagctct ttcacactct tcagtttctt ggacttgccc ttttccactt
      781 tggccaccac cagcacagaa taggccacgg tggggctgtc gaagccgccg tacttcttag
      841 ggtcccagtc cttctttctg gcgatcagct tatcgctgtt cctcttgggc aggatagact
      901 ctttgctgaa gccgcctgtc tgcacctcgg tctttttcac gatattcact tggggcatgc
      961 tcagcacttt ccgcacggtg gcaaaatccc ggcccttatc ccacacgatc tccccggttt
     1021 cgccgtttgt ctcgatcaga ggccgcttcc ggatctcgcc gttggccagg gtaatctcgg
     1081 tcttgaaaaa gttcatgatg ttgctgtaga agaagtactt ggcggtagcc ttgccgattt
     1141 cctgctcgct cttggcga
//
