LOCUS       sequence-115331-         751 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses intein-Cas9(C574) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Davis KM, Pattanayak V, Thompson DB, Zuris JA, Liu DR
  TITLE     Small molecule-triggered Cas9 protein with improved genome-editing 
            specificity.
  JOURNAL   Nat Chem Biol. 2015 May;11(5):316-8. doi: 10.1038/nchembio.1793. 
            Epub 2015 Apr 6.
  PUBMED    25848930
REFERENCE   2  (bases 1 to 751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.1793"; journalName: "Nat Chem Biol"; date: 
            "2015-05"; volume: "11"; issue: "5"; pages: "316-8"
COMMENT     Sequence Label: pKMD106h - intein-Cas9(C574)
FEATURES             Location/Qualifiers
     source          1..751
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ctggagtgta cacatttctg tccagcaccc tgaagtctct ggaagagaag gaccatatcc
       61 accgagccct ggacaagatc acggacactt tgatccacct gatggccaag gcaggcctga
      121 ccctgcagca gcagcaccag cggctggccc agctcctcct catcctctcc cacatcaggc
      181 acatgagtaa caaaggaatg gagcatctgt acagcatgaa gtacaagaac gtggtgcccc
      241 tctatgacct gctgctggag atgctggacg cccaccgcct acatgcgggt ggttctggtg
      301 ctagccgcgt gcaggcgttc gcggatgccc tggatgacaa attcctgcac gacatgctgg
      361 cggaaggact ccgctattcc gtgatccgag aagtgctgcc aacgcggcgg gcacgaacgt
      421 tcgacctcga ggtcgaggaa ctgcacaccc tcgtcgccga aggggttgtc gtgcacaact
      481 gcttcgattc tgtcgagatc tccggggtag aagatcgatt taatgcgtca cttggtacgt
      541 atcatgacct cctaaagata attaaagata aggacttcct ggataacgaa gagaatgaag
      601 atatcttaga agatatagtg ttgactctta ccctctttga agatcgggaa atgattgagg
      661 aaagactaaa aacatacgct cacctgttcg acgataaggt tatgaaacag ttaaagaggc
      721 gtcgctatac gggctgggga cgattgtcgc g
//
