LOCUS       sequence-11543-e        7960 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7960)
  AUTHORS   Williams KJ, Joyce G, Robertson BD
  TITLE     Improved mycobacterial tetracycline inducible vectors.
  JOURNAL   Plasmid. 2010 Sep;64(2):69-73. doi: 10.1016/j.plasmid.2010.04.003. 
            Epub 2010 Apr 29.
  PUBMED    20434484
REFERENCE   2  (bases 1 to 7960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.plasmid.2010.04.003"; journalName: "Plasmid"; date: 
            "2010-09"; volume: "64"; issue: "2"; pages: "69-73"
COMMENT     Sequence Label: pKW08-Lx
FEATURES             Location/Qualifiers
     source          1..7960
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      14..41
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(2903..2919)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2904..2920)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     rep_origin      7125..7713
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7614..7633
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7867..7884
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tgattagcta agcagaaggc catcctgacg gatggccttt ttgcgtttaa tactgtttaa
       61 acctctagat cacgattcgc tcgaggtcaa tgacgctgtg cgggagtgag agaatccgtc
      121 gatcagtgct gtcaggccga aatcgaattc ccggtcgcgt tcaggaacgc tcgcattgcc
      181 gacctctgcg gtttgctcct ccagtacgac accgacggtg tatcggctga ccgcgatgag
      241 catgctgatg gctgtctgtt catcgaatcc ctgagacacg agaagcccga cttgctgacc
      301 ggaagcctca gctccttcgt cggtgtcgtc gccggatggt ctgcggtggt gctcagcgtg
      361 gagacgcgca ccgtcacgga ccgcgtgtag tgcgtggcgg aaacttcttg cgtttcgcaa
      421 gagaaatgcg tcccatttct cgtcggactc ggggaaggaa gcgtgatgct ctcggtcaag
      481 cacgtcgctc gccagcgctg cgaggagttc ggccttcgtg cggaagtgcc agtagaggcc
      541 gggctgctgt acctgtaagt gagccgccag cgcgcgagtg gtgaagccat cgagcccagt
      601 ctcgtcgagc acctgccggg ccccgagcaa cacggacgtg cggtcgagac gcttccggtg
      661 gtgagtcata gttgcacttt atcatcgata actttatctt agataaagtg actgctcgct
      721 actctcatct gactgctcgc tactctcatc gtggaatcct gacaggatcc ggaggaaatg
      781 ttatgaaatt tggaaacttc ctcctcactt atcagccacc tgagctatct cagaccgaag
      841 tgatgaagcg attggttaat ctgggcaaag cgtctgaagg ttgtggcttc gacaccgttt
      901 ggttgctaga gcaccacttc actgaatttg ggttgttagg gaatccttat gttgctgccg
      961 cacacctatt aggtgcgaca gaaacgctca acgttggcac tgcagctatc gtattgccga
     1021 ctgcccatcc ggttcgacaa gcagaagacg taaacctact ggatcaaatg tcaaaaggac
     1081 gattccgttt tggtatttgt cgcggtttgt acgataaaga ttttcgtgtc tttggtacag
     1141 acatggataa cagccgagcc ttaatggact gttggtatga cttgatgaaa gaaggcttca
     1201 atgaaggcta tatcgcggcg gataacgaac atattaagtt cccgaaaatc caactgaatc
     1261 catcggctta cacacaaggt ggtgctcctg tttatgtcgt cgcggagtca gcatcaacga
     1321 cagaatgggc tgcagagcgt ggcctaccaa tgattctaag ctggatcatc aacactcacg
     1381 agaagaaagc gcagcttgat ctttacaacg aagtcgcgac tgaacatggc tacgatgtga
     1441 ctaagattga ccactgtttg tcttacatca cctccgtcga tcatgactca aatagagcca
     1501 aagatatttg ccgcaacttc ttgggccatt ggtacgactc atacgtgaat gccaccaaga
     1561 tttttgacga ctctgaccaa acaaaaggtt acgacttcaa taaaggtcaa tggcgtgatt
     1621 ttgtgttgaa aggccacaaa gacaccaatc gccgaattga ttacagctac gaaatcaacc
     1681 cagtagggac gcctgaagag tgtatcgcga ttatccagca agatattgat gcgacgggta
     1741 ttgacaatat ttgttgtggt tttgaagcaa acggttctga agaagaaatt atcgcatcta
     1801 tgaagctatt ccagtctgat gtgatgccat atctcaaaga aaaacagtaa ttaatatttt
     1861 ctaaaaggaa agagacatga aatttggatt attcttcctc aattttatga actcaaagcg
     1921 ttcttctgat caagtcatcg aagaaatgtt agataccgca cattacgtag atcagttgaa
     1981 gtttgacacg ttggctgttt acgaaaacca tttctcgaac aatggtgtgg ttggtgcccc
     2041 actaacagtg gctggttttt tacttggtat gacaaagaac gccaaagtgg cttcgttgaa
     2101 tcacgtcatt accacgcatc atccagtacg tgtggcggaa gaagcgtgtc tacttgacca
     2161 aatgagtgaa ggccgttttg cctttggctt tagtgattgt gaaaagagtg cagatatgcg
     2221 cttctttaat cgaccaacgg attctcagtt tcagttgttc agtgagtgtc acaagatcat
     2281 caatgattca ttcactactg ggtactgcca tccaaacaat gatttttata gttttcctaa
     2341 aatctccgtt aacccacacg cgttcactga aggcggtcct gcgcaatttg tgaatgcgac
     2401 gagcaaagaa gtggttgaat gggcggctaa gttagggctt ccactcgtgt ttagatggga
     2461 cgactcaaac gctcaaagaa aagaatacgc cggtttgtac cacgaagttg ctcaggcaca
     2521 tggtgtcgat gttagtcagg ttcgacacaa gctgacgctg ctggtcaacc aaaatgtaga
     2581 tggtgaagca gcaagggcag aagctcgcgt gtatttggaa gagtttgtcc gtgaatctta
     2641 ctcaaatacc gactttgagc aaaaaatggg agagctgttg tcagaaaatg ccatcggtac
     2701 ttatgaagaa agtactcagg cagcgcgagt tgcgattgag tgttgtggtg ccgcggacct
     2761 attgatgtct tttgagtcga tggaagataa agcgcagcaa agagcggtta tcgatgtggt
     2821 aaacgccaac atcgtcaaat accactcgta aaagctttag ctaattaatt ggggacccta
     2881 gaggtcccct tttttatttt atcgataccg tcgacctcga gggggggccc gccggggatc
     2941 atcgagccga gaacgttatc gaagttggtc atgtgtaatc ccctcgtttg aactttggat
     3001 taagcgtaga tacacccttg gacaagccag ttggattcgg agacaagcaa attcagcctt
     3061 aaaaagggcg aggcctgcgg tggtggaaca ccgcagggcc tctaaccgct cgacgcgctg
     3121 caccaaccag cccgcgaacg gctggcagcc agcgtaaggc gcggctcatc gggcggcgtt
     3181 cgccacgatg tcctgcactt cgagccaagc ctcgaacacc tgctggtgtg cacgactcac
     3241 ccggttgttg acaccgcgcg cggccgtgcg ggctcggtgg ggcggctgtg tcgcccttgc
     3301 cagcgtgagt agcgcgtacc tcacctcgcc caacaggtcg cacacagccg attcgtacgc
     3361 cataaagcca ggtgagccca ccagctccgt aagttcgggc gctgtgtggc tcgtacccgc
     3421 gcattcaggc ggcagggggt ctaacgggtc taaggcggcg tgtacggccg ccacagcggc
     3481 tctcagcggc ccggaaacgt cctcgaaacg acgcatgtgt tcctcctggt tggtacaggt
     3541 ggttgggggt gctcggctgt cgctggtgtt ccaccaccag ggctcgacgg gagagcgggg
     3601 gagtgtgcag ttgtggggtg gcccctcagc gaaatatctg acttggagct cgtgtcggac
     3661 catacaccgg tgattaatcg tggtctacta ccaagcgtga gccacgtcgc cgacgaattt
     3721 gagcagctct ggctgccgta ctggccgctg gcaagcgacg atctgctcga ggggatctac
     3781 cgccaaagcc gcgcgtcggc cctaggccgc cggtacatcg aggcgaaccc aacagcgctg
     3841 gcaaacctgc tggtcgtgga cgtagaccat ccagacgcag cgctccgagc gctcagcgcc
     3901 cgggggtccc atccgctgcc caacgcgatc gtgggcaatc gcgccaacgg ccacgcacac
     3961 gcagtgtggg cactcaacgc ccctgttcca cgcaccgaat acgcgcggcg taagccgctc
     4021 gcatacatgg cggcgtgcgc cgaaggcctt cggcgcgccg tcgacggcga ccgcagttac
     4081 tcaggcctca tgaccaaaaa ccccggccac atcgcctggg aaacggaatg gctccactca
     4141 gatctctaca cactcagcca catcgaggcc gagctcggcg cgaacatgcc accgccgcgc
     4201 tggcgtcagc agaccacgta caaagcggct ccgacgccgc tagggcggaa ttgcgcactg
     4261 ttcgattccg tcaggttgtg ggcctatcgt cccgccctca tgcggatcta cctgccgacc
     4321 cggaacgtgg acggactcgg ccgcgcgatc tatgccgagt gccacgcgcg aaacgccgaa
     4381 ttcccgtgca acgacgtgtg tcccggaccg ctaccggaca gcgaggtccg cgccatcgcc
     4441 aacagcattt ggcgttggat cacaaccaag tcgcgcattt gggcggacgg gatcgtggtc
     4501 tacgaggcca cactcagtgc gcgccagtcg gccatctcgc ggaagggcgc agcagcgcgc
     4561 acggcggcga gcacagttgc gcggcgcgca aagtccgcgt cagccatgga ggcattgcta
     4621 tgagcgacgg ctacagcgac ggctacagcg acggctacaa ccggcagccg actgtccgca
     4681 aaaagcggcg cgtgaccgcc gccgaaggcg ctcgaatcac cggactatcc gaacgccacg
     4741 tcgtccggct cgtggcgcag gaacgcagcg agtggctcgc cgagcaggct gcacgccgcg
     4801 aacgcatccg cgcctatcac gacgacgagg gccactcttg gccgcaaacg gccaaacatt
     4861 tcgggctgca tctggacacc gttaagcgac tcggctatcg ggcgaggaaa gagcgtgcgg
     4921 cagaacagga agcggctcaa aaggcccaca acgaagccga caatccaccg ctgttctaac
     4981 gcaattgggg agcgggtgtc gcgggggttc cgtggggggt tccgttgcaa cgggtcggac
     5041 aggtaaaagt cctggtagac gctagttttc tggtttgggc catgcctgtc tcgttgcgtg
     5101 tttcgttgcg cccgttttga ataccagcca gacgagacgg ggttctacga atcttggtcg
     5161 ataccaagcc atttccgctg aatatcgggg agctcaccgc cagaatcggt ggttgtggtg
     5221 atgtacgtgg cgaactccgt tgtagtgcct gtggtggcat ccgtggccac tctcgttgca
     5281 cggttcgttg tgccgttaca ggccccgttg acagctcacc gaacgtagtt aaaacatgct
     5341 ggtcaaacta ggtttaccaa cgatacgagt cagctcatct agggccagtt ctaggcgttg
     5401 ttcgttgcgc ggttcgttgc gcatgtttcg tgtggttgct agatggctcc gcaaccacac
     5461 gcttcgaggt tgagtgcttc cagcacgggc gcgatccaga agaacttcgt cgtgcgactg
     5521 tcctcgttga tccttgccga gctgggatgg aagctcggcc gaccaccctg gaggagatga
     5581 tcgaggatgc cagggccttt cacgcccgcc gctgctgagc gtccgccgcc gggcccgcac
     5641 cgccgtcggc cggcccgctc cgggctcgca gcagcgggct tcggcgcggg cccggggctc
     5701 ccgagcgcgg gcggggctcc gggcggccgc cgggggccgg gggcggcgcc gggcggcccg
     5761 gggcgtcagg cgccgggggc ggtgtccggc ggcccccaga ggaactgcgc cagttcctcc
     5821 ggatcggtga agccggagag atccagcggg gtctcctcga acacctcgaa gtcgtgcagg
     5881 aaggtgaagg cgagcagttc gcgggcgaag tcctcggtcc gcttccactg cgccccgtcg
     5941 agcagcgcgg ccaggatctc gcggtcgccc cggaaggcgt tgagatgcag ttgcaccagg
     6001 ctgtagcggg agtctcccgc atagacgtcg gtgaagtcga cgatcccggt gacctcggtc
     6061 gcggccaggt ccacgaagat gttggtcccg tgcaggtcgc cgtggacgaa ccggggttcg
     6121 cggccggcca gcagcgtgtc cacgtccggc agccagtcct ccaggcggtc cagcagccgg
     6181 ggcgagaggt agccccaccc gcggtggtcc tcgacggtcg ccgcgcggcg ttcccgcagc
     6241 agttccggga agacctcgga atggggggtg agcacggtgt tcccggtcag cggcaccctg
     6301 tgcagccggc cgagcacccg gccgagttcg cgggccaggg cgagcagcgc gttccggtcg
     6361 gtcgtgccgt ccatcgcgga ccgccaggtg gtgccggtca tccggctcat caccaggtag
     6421 ggccacggcc aggctccggt gccgggccgc agctcgccgc ggccgaggag gcggggcacc
     6481 ggcaccgggg cgtccgccag gaccgcgtac gcctccgact ccgacgcgag gctctccgga
     6541 ccgcaccagt gctcgccgaa cagcttgatc accgggtcgg gctcgccgac cagtacgggg
     6601 ttggtgctct cgccgggcac ccgcagcacc ggcggcaccg gcagcccgag ctcctccagg
     6661 gctcggcggg ccagcggctc ccagaattcc tggtcgttcc gcaggctcgc gtaggaatca
     6721 tccgaatcaa tacggtcgag aagtaacagg gattcttgtg tcacagcgga cctctattca
     6781 cagggtacgg gccggcttaa ttccgcacgg ccggtcgcga cacggcctgt ccgcaccgcg
     6841 gtcaggcgtt gacgatgacg ggctggtcgg ccacgtccgg ggagtcaggc aactatggat
     6901 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     6961 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     7021 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     7081 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     7141 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     7201 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     7261 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     7321 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     7381 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     7441 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     7501 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     7561 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     7621 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     7681 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     7741 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     7801 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     7861 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     7921 cccgcgcgtt ggccgattca ttaatgcagc tagaactagt
//
