LOCUS       sequence-115503-         997 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Kim JB, Sarraf P, Wright M, Yao KM, Mueller E, Solanes G, Lowell BB,
            Spiegelman BM
  TITLE     Nutritional and insulin regulation of fatty acid synthetase and 
            leptin gene expression through ADD1/SREBP1.
  JOURNAL   J Clin Invest. 1998 Jan 1. 101(1):1-9.
  PUBMED    9421459
REFERENCE   2  (bases 1 to 997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 1998 Jan 1. 101(1):1-9."
COMMENT     Sequence Label: FAS promoter luciferase
FEATURES             Location/Qualifiers
     source          1..997
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     3..22
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3..21
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    203..337
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(240..259)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     294..313
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(688..706)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 tgtaatacga ctcactatag ggcgaattgg gcccgacgtc gcatgctcct ctagagcttg
       61 cagatctgcg actctagagg atctgcgact ctagaggatc ataatcagcc ataccacatt
      121 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
      181 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
      241 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
      301 gtccaaactc atcaatgtat cttatcatgt ctggatcccc gggtaccggg ggctgcgcgt
      361 tccttgtgct ccagcgcgcg cctgtgcagg gtcccggctg ggggcggcgc gcgcgggcat
      421 caccccaccg acggcggcgc gccgggtccc ggggcgcagc cccgacgctc attggcctgg
      481 gcggcgcagc caagctgtca gcccatgtgg cgtggccgcg cggggatggc cgcggtttaa
      541 atagcgccgg cgcggcctag agggagccag agagacggca gcagcgtccc gtaagcttgg
      601 cattccggta ctgttggtaa aatggaagac gccaaaaaca taaagaaagg cccggcgcca
      661 ttctatcctc tagaggatgg aaccgctgga gagcaactgc ataaggctat gaagagatac
      721 gccctggttc ctggaacaat tgcttttaca gatgcacata tcgaggtgaa catcacgtac
      781 gcggaatact tcgaaatgtc cgttcggttg gcagaagcta tgaaacgata tgggctgaat
      841 acaaatcaca gaatcgtcgt atgcagtgaa aactctcttc aattctttat gccggtgttg
      901 ggcgcgttat ttatcggagt tgcagttgcg cccgcgaacg acatttataa tgaacgtgaa
      961 ttgctcaaca gtatgaacat ttcgcagcct accgtag
//
