LOCUS       sequence-115515-        1020 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses PTS2-YNL in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Takai A, Nakano M, Saito K, Haruno R, Watanabe TM, Ohyanagi T, Jin 
            T, Okada Y, Nagai T
  TITLE     Expanded palette of Nano-lanterns for real-time multicolor 
            luminescence imaging.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Apr 7;112(14):4352-6. doi: 
            10.1073/pnas.1418468112. Epub 2015 Mar 23.
  PUBMED    25831507
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1418468112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-04-7- 7"; volume: "112"; issue: "14"; pages: "4352-6"
COMMENT     Sequence Label: pYNL-N1_PTS2
FEATURES             Location/Qualifiers
     source          1..1020
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      164..173
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(170..172,173..175,176..688)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(215..236)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(476..495)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     823..844
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggactcagat
       61 ctcgagctca agcttcgaat tcgccaccat gcagagattg caagtggtcc tagggcacct
      121 ccgaggaccg tcgacggtac cgcgggcccg ggatccaccg gtcgccacca tggtgagcaa
      181 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
      241 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
      301 cctgaagctg atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
      361 cctgggctac ggcctgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt
      421 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
      481 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
      541 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
      601 caactacaac agccacaacg tctatatcac cgccgacaag cagaagaacg gcatcaaggc
      661 caacttcaag atccgccaca acatcgagga cggcggcgtg cagctcgccg accactacca
      721 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcta
      781 ccagtccaag ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
      841 cgtgaccgcc gccgggggta ccaaggtgta cgaccccgag cagaggaaga ggatgatcac
      901 cggcccccag tggtgggcca ggtgcaagca gatgaacgtg ctggacagct tcatcaacta
      961 ctacgacagc gagaagcacg ccgagaacgc cgtgatcttc ctgcacggca acgccactag
//
