LOCUS       sequence-115561-         704 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  ER calcium sensor for lentivirus production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Henderson MJ, Baldwin HA, Werley CA, Boccardo S, Whitaker LR, Yan X,
            Holt GT, Schreiter ER, Looger LL, Cohen AE, Kim DS, Harvey BK
  TITLE     A Low Affinity GCaMP3 Variant (GCaMPer) for Imaging the Endoplasmic 
            Reticulum Calcium Store.
  JOURNAL   PLoS One. 2015 Oct 9;10(10):e0139273. doi: 
            10.1371/journal.pone.0139273. eCollection 2015.
  PUBMED    26451944
REFERENCE   2  (bases 1 to 704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-10-9"; volume: "10(10):e0139273. doi"; pages: " 
            10.1371/journal.pone.0139273. eCollection 2015"
COMMENT     Sequence Label: pMOS003-lenti-CMV-GCaMPer
FEATURES             Location/Qualifiers
     source          1..704
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(72..512)
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKGGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDAGGDGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKGGNGYISAAEL
                     RHVMTNLGEKLTDEEVDEMIREADIDGGGQVNYEEFVQMMTAK"
     primer_bind     628..647
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 attttgtaat ccagaggttg attatcgata agcttgatat cgaattccta caactcatct
       61 ttttctgctg tcttcgctgt catcatttgt acaaactctt cgtagtttac ctgaccaccc
      121 ccatcgatgt ctgcttccct gatcatttca tcaacctctt catctgttaa cttctctcca
      181 aggtttgtca tcacgtggcg aagctctgct gcactgatgt agccattgcc acccttatca
      241 aacacacgga acgcttctct aatttcttct tcactgtctg tgtctttcat ttttcttgcc
      301 atcattgtca ggaactcagg gaagtcgatt gtgccgtcac cgccggcatc tacttcattg
      361 atcatgtcct gcagctctgc ttctgtgggg ttctgcccca gagaccgcat caccgtcccc
      421 agctccttgg ttgttattgt cccatccccg cccttgtcaa atagggagaa agcctcttta
      481 aattctgcga tctgctcttc agtcagttgg tcacgcgtgt tgtactccag cttgtgcccc
      541 aggatgttgc cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt caccagggtg
      601 tcgccctcga acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg
      661 gtgcgctcct ggatgtagcc ttcgggcatg gcggacttga agaa
//
