LOCUS       sequence-115636-         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of PhiC31o recombinase from the broadly active 
            CAG promoter/enhancer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Ohtsuka M, Miura H, Mochida K, Hirose M, Hasegawa A, Ogura A, 
            Mizutani R, Kimura M, Isotani A, Ikawa M, Sato M, Gurumurthy CB
  TITLE     One-step generation of multiple transgenic mouse lines using an 
            improved Pronuclear Injection-based Targeted Transgenesis (i-PITT).
  JOURNAL   BMC Genomics. 2015 Apr 9;16:274. doi: 10.1186/s12864-015-1432-5.
  PUBMED    25887549
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Genomics."; date: "2015-04-9"; volume: "16:274. doi"; pages: " 
            10.1186/s12864-015-1432-5"
COMMENT     Sequence Label: pBCW
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     29..48
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      76..85
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat
       61 tttggcaaag aattcgccac catggatacc tacgccggag cctacgacag acagagccgg
      121 gagagagaga acagcagcgc cgccagcccc gccacccaga gaagcgccaa cgaggataag
      181 gccgccgatc tgcagagaga ggtggagagg gacggcggca gattcagatt tgtgggccac
      241 ttcagcgagg cccctggcac cagcgccttc ggcaccgccg agagacccga gttcgagaga
      301 atcctgaacg agtgtagggc cggcaggctg aacatgatca tcgtgtacga cgtgtcccgg
      361 ttcagcaggc tgaaggtgat ggacgccatc cctatcgtgt ccgagctgct ggccctgggc
      421 gtgaccatcg tgtccaccca ggaaggcgtc tttagacagg gcaacgtgat ggacctgatc
      481 cacctgatca tgaggctgga cgccagccac aaggagagca gcctgaagag cgccaagatc
      541 ctggacacca agaacctgca gagggagctg ggcggctatg tgggcggcaa ggccccctac
      601 ggcttcgagc tggtgtccga gaccaaggag atcacccgga acggcaggat ggtgaacgtg
      661 gtgatcaaca agctggccca cagcaccacc cccctgaccg gccccttcga gtttgagccc
      721 gacgtgatca ggtggtggtg gcgggagatc aagacccaca agcacctgcc tttcaagccc
      781 ggcagccagg ccgccat
//
