LOCUS       sequence-115673-        3582 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression / in vitro transcription.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Feike AC, Rachor K, Gentzel M, Schambony A
  TITLE     Wnt5a/Ror2-induced upregulation of xPAPC requires xShcA.
  JOURNAL   Biochem Biophys Res Commun. 2010 Oct 1;400(4):500-6. doi: 
            10.1016/j.bbrc.2010.08.074. Epub 2010 Aug 21.
  PUBMED    20732301
REFERENCE   2  (bases 1 to 3582)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bbrc.2010.08.074"; journalName: "Biochem Biophys Res 
            Commun"; date: "2010-10-1- 1"; volume: "400"; issue: "4"; pages: 
            "500-6"
COMMENT     Sequence Label: pCS2 xRor2-EGFP
FEATURES             Location/Qualifiers
     source          1..3582
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(2815..2817,2818..2820,2821..3531)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2815..2817,2818..2820,2821..3531)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2860..2881)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3121..3140)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3468..3489
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 atgtccagga ccaggagcca gaatgggggg attggatgtg ccctgctcgg gcttctcgtg
       61 gctgccatac tgctgcctgt acaagcttca ggtgaaatgg aaataccaga cttgaatgac
      121 ccactgggac aaatggaaag ccacgacagg ctcggagcaa ctcctagagg atactatttg
      181 acgtttctag aacctgtgaa caatatcaca attgtgcaag gtcaggcagc aacactccat
      241 tgcaaagtgt ccgggaatcc actccccaat gtaaagtggc tgaagaacga tgcacctgtg
      301 gttcaggagc ccaagagaat taccattagg aaaacagact acggctcacg attacggata
      361 caagacctgg acaccaccga cactggctat tatcagtgtg tcgccaccaa tgggataaag
      421 accattactg caactggagt cctctttgtt aggcttggac caacaaacag ccctaacccg
      481 aacatccagg atgactacca caaagatggg ttttgtcagc cctacagggg aattgcctgc
      541 gcaagattca ttggcaacag gaccatatat gtcgactcac ttcagatgca aggggaaatc
      601 gaaaaccgaa ttacagctgc ctttacaatg attggcactt ccacccatct ctctgaccag
      661 tgctctcagt ttgcaatccc atcattttgc cacttcgtgt tccctctctg tgatgatcaa
      721 acttccaagc caagagagct gtgcagggat gagtgtgaag tgctggaaaa tgacctgtgc
      781 aggcaggagt acaacatcgc ccgctccaat cccctcattc tcatgcagct tcacctacca
      841 aactgcgaag aactgccatt gcctgaaagt cctgaagctg ccaactgcat gaggattggg
      901 atcccagtgg agaagcttaa cagatatcag cagtgttaca atgggactgg aactgattac
      961 agaggatctg tcagcgtcac taagtctggc catcagtgcc agccatggag tcatcaggtt
     1021 ccccatagtc attcactatc caatgcggat taccctgaaa ttggtggagg ccactcttac
     1081 tgcagaaatc caggaggcca gatggagggt ccttggtgtt tcacacagaa taaaaatgtc
     1141 cgcatggagc tatgcgatat tcctgcttgc cgtacacgtg acaacacaaa gatggagatt
     1201 ctctacatct tggtccccag cattgcaatc ccactggtca tcgcctgctt ctttttctta
     1261 gtatgcatgt gccgaaataa acagaaagca gaaggttcta caccgcagcg ccgccaactc
     1321 atggcctctc caagccagga catggaaatg ccccttatga atcagcacaa acagcagcca
     1381 aaagtaaaag agataaacct atcaactgta aggttcatgg aagagctagg tgaagatcgt
     1441 tttggaaaag tttacaaggg acatctcttt ggtaccactc caggggaaca gactcaaact
     1501 gttgccatca aaacacttaa agacaaagtt gaagttgctc ttcgtgaaga attcaaacat
     1561 gaggccatga tgcgatctag gcttcagcac ccaaatatag tttgtctgat tgggacagtg
     1621 acaaaggagc agcccatgag catgatattt agctattctc ctcttagtga tctgcacgag
     1681 ttcttggtca tgagatcacc acattcagat gtaggcagca cagatgacga taagactgtt
     1741 aaatccaccc tagagcccgc tgattttctt catattgtga cccagatagc atcaggaatg
     1801 gaatttcttt caagtcacca tgttgttcat aaagatttgg ctgccagaaa tgttttggtg
     1861 ttcgataaac tcagcattaa aatttcagat ctcggcctgt tccgggaagt atatgctgca
     1921 gattactata aactgatggg aaactccatg cttccgatta gatggatgtc tccagaagcg
     1981 atcacatatg gaaagtgttc tgttgattca gatatttggt cctatggggt cgtggtgtgg
     2041 gaaatcttca gctacgggct gcaaccgtat tgtgggtatt ccaaccagga tgtcattgaa
     2101 atgatccgaa atcgccaggt gctgctctgc cctgatgact gtcctgcatg gatatacact
     2161 ctaatgctgg agtgctggag tgaatttcct gctagaagac caagatttaa ggacatacac
     2221 acacggctaa gaacatggga aaatatgtcc aactataata gctctgcaca aacatctggg
     2281 gcaagtaata ctacccagac aagttctcta agcacaagcc cagtaagcaa tgttagcaat
     2341 gctaggtatg ttgggccaaa gccaaaaact cagccattcc aacagccaca gtttttgcaa
     2401 atgaaaggac aaattagacc tatggttcct caacctcaac tctacattcc agttaatggg
     2461 tatcaacaga tggctgctta tttttaccca gttcagatac caatgcaaat ggctccacaa
     2521 caaatgccac ctcagatcat acccaaacct ggatcccacc acagtggaag tggttccacc
     2581 agtacaggat atgtcacaac agcgccctca aataattcca tggctgacag ggttgctctc
     2641 cttgcagatg gggcagatga agcacagtta acggcagaag atatgtctcc aaatcctggt
     2701 caggaagaag aaggttctgt tccagaaaca gaattgctgg gtgacaatga cacatctcaa
     2761 ttagatgcaa cagatattca gtcagagact gctagcgcta ccggtcgcca ccggatggtg
     2821 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     2881 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     2941 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     3001 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     3061 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     3121 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     3181 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     3241 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     3301 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     3361 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     3421 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     3481 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtccggactc
     3541 agatctcgag cctctagaac tatagtgagt cgtattacgt ag
//
