LOCUS       sequence-115784-         597 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Song R, Walentek P, Sponer N, Klimke A, Lee JS, Dixon G, Harland R, 
            Wan Y, Lishko P, Lize M, Kessel M, He L
  TITLE     miR-34/449 miRNAs are required for motile ciliogenesis by repressing
            cp110.
  JOURNAL   Nature. 2014 Jun 5;510(7503):115-20. doi: 10.1038/nature13413.
  PUBMED    24899310
REFERENCE   2  (bases 1 to 597)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2014-06-5- 5"; volume: "510"; issue: "7503"; pages:
            "115-20"
COMMENT     Sequence Label: pGL3-Cp110-3'UTR
FEATURES             Location/Qualifiers
     source          1..597
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      36..45
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(75..271)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      89..224
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     misc_feature    326..417
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(358..377)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    431..479
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 tggcgccggg cctttcttta tgtttttggc gtcttccatg gtggctttac caacagtacc
       61 ggaatgccaa gctttttgca aaagcctagg cctccaaaaa agcctcctca ctacttctgg
      121 aatagctcag aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat tagtcagcga
      181 tggggcggag aatgggcgga actgggcgga gttaggggcg ggatgggcgg agttaggggc
      241 gggactatgg ttgctgacta attgagatgc agatcgcaga tctcgagccc gggctagcac
      301 gcgtaagagc tcggtaccta tcgatagaga aatgttctgg cacctgcact tgcactgggg
      361 acagcctatt ttgctagttt gttttgtttc gttttgtttt gatggagagc gtatgttagt
      421 actatcgatt cacacaaaaa accaacacac agatgtaatg aaaataaaga tattttattg
      481 cggccgctcc aagtacctcc cgtaccttaa tattacttac ttatcatggt agcttgggct
      541 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctga
//
