LOCUS       sequence-115785-         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Song R, Walentek P, Sponer N, Klimke A, Lee JS, Dixon G, Harland R, 
            Wan Y, Lishko P, Lize M, Kessel M, He L
  TITLE     miR-34/449 miRNAs are required for motile ciliogenesis by repressing
            cp110.
  JOURNAL   Nature. 2014 Jun 5;510(7503):115-20. doi: 10.1038/nature13413.
  PUBMED    24899310
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2014-06-5- 5"; volume: "510"; issue: "7503"; pages:
            "115-20"
COMMENT     Sequence Label: pGL3-Cp110-3'UTR
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        61..257
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      108..243
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      287..296
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(359..377)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 ttctctatcg ataggtaccg agctcttacg cgtgctagcc cgggctcgag atctgcgatc
       61 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
      121 ctccgcccag ttccgcccat tctccgcccc atcgctgact aatttttttt atttatgcag
      181 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
      241 gcctaggctt ttgcaaaaag cttggcattc cggtactgtt ggtaaagcca ccatggaaga
      301 cgccaaaaac ataaagaaag gcccggcgcc attctatccg ctggaagatg gaaccgctgg
      361 agagcaactg cataaggcta tgaagagata cgccctggtt cctggaacaa ttgcttttac
      421 agatgcacat atcgaggtgg acatcactta cgctgagtac ttcgaaatgt ccgttcggtt
      481 ggcagaagct atgaaacgat atgggctgaa tacaaatcac agaatcgtcg tatgcagtga
      541 aaactctctt caattcttta tgccggtgtt gggcgcgtta tttatcggag ttgcagttgc
      601 gcccgcgaac gacatttata atgaacgtga attgctcaac agtatgggca tttcgcagcc
      661 taccgtggtg ttcgtttcca aaaaggggtt gcaaaaaatt ttgaacgtgc aaaaaaagct
      721 cccaatcatc caaaaaatta ttatcatgga ttctaaaacg gattaccagg gatttcagtc
      781 gatgtacacg ttcgtcacat ctcatctacc tcccgg
//
