LOCUS       sequence-115822-         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Pten Luciferase reporter .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Olive V, Bennett MJ, Walker JC, Ma C, Jiang I, Cordon-Cardo C, Li 
            QJ, Lowe SW, Hannon GJ, He L
  TITLE     miR-19 is a key oncogenic component of mir-17-92.
  JOURNAL   Genes Dev. 2009 Dec 15. 23(24):2839-49.
  PUBMED    20008935
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2009 Dec 15. 23(24):2839-49."
COMMENT     Sequence Label: Luc-Pten-3'UTR
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        50..801
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     regulatory      830..839
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atcgataggt accgagctct tacgcgtgct agcccgggct cgagatctaa atgagtcttc
       61 ggacctcgcg ggggccgctt aagcggtggt tagggtttgt ctgacgcggg gggaggggga
      121 aggaacgaaa cactctcatt cggaggcggc tcggggtttg gtcttggtgg ccacgggcac
      181 gcagaagagc gccgcgatcc tcttaagcac ccccccgccc tccgtggagg cgggggtttg
      241 gtcggcgggt ggtaactggc gggccgctga ctcgggcggg tcgcgcgccc cagagtgtga
      301 ccttttcggt ctgctcgcag acccccgggc ggcgccgccg cggcggcgac gggctcgctg
      361 ggtcctaggc tccatgggga ccgtatacgt ggacaggctc tggagcatcc gcacgactgc
      421 ggtgatatta ccggagacct tctgcgggac gagccgggtc acgcggctga cgcggagcgt
      481 ccgttgggcg acaaacacca ggacggggca caggtacact atcttgtcac ccggaggcgc
      541 gagggactgc aggagcttca gggagtggcg cagctgcttc atccccgtgg cccgttgctc
      601 gcgtttgctg gcggtgtccc cggaagaaat atatttgcat gtctttagtt ctatgatgac
      661 acaaaccccg cccagcgtct tgtcattggc gaattcgaac acgcagatgc agtcggggcg
      721 gcgcggtccc aggtccactt cgcatattaa ggtgacgcgt gtggcctcga acaccgagcg
      781 accctgcagc gacccgctta aagcttggca ttccggtact gttggtaaag ccaccatgga
      841 agacgccaaa aacataaaag
//
