LOCUS       sequence-115918-        3792 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  yeast ORF Nsr1 in the Gateway entry vector pDONR221.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   McCann KL, Charette JM, Vincent NG, Baserga SJ
  TITLE     A protein interaction map of the LSU processome.
  JOURNAL   Genes Dev. 2015 Apr 15;29(8):862-75. doi: 10.1101/gad.256370.114. 
            Epub 2015 Apr 15.
  PUBMED    25877921
REFERENCE   2  (bases 1 to 3792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.256370.114"; journalName: "Genes Dev"; date: 
            "2015-04-15- 15"; volume: "29"; issue: "8"; pages: "862-75"
COMMENT     Sequence Label: pDONR221 Nsr1
FEATURES             Location/Qualifiers
     source          1..3792
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1917..2016)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2033..2052)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2034..2052)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2057..2073)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2057..2073)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2175..2194)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2186..2995
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2253..2272)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3142..3730
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3631..3650
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctac aaaatggcta agactactaa agtaaaaggt aacaagaagg aagttaaggc
      721 ttccaaacaa gccaaagaag aaaaagctaa ggccgtctct tcctcttcct ccgaatcttc
      781 atcctcatct tcatcttcat ctgaatctga atctgagtct gagtctgaat ctgaatcttc
      841 atcttcatct tcatcctctg attctgaatc ctcttcttca tcgtcttctg acagcgaaag
      901 tgaagctgaa accaagaagg aagaatccaa ggattcctct tcctcttcct ctgactcttc
      961 ttccgacgaa gaagaagaag aagaaaaaga agaaaccaag aaggaagaat caaaagaatc
     1021 ttctagctct gattcatcct catcttcatc ttctgatagc gaaagcgaaa aggaagagtc
     1081 taacgataag aaacgtaaat ctgaggacgc cgaagaagaa gaagacgaag agtcttccaa
     1141 caagaagcaa aaaaatgaag aaaccgaaga accagctact attttcgttg gtagactatc
     1201 gtggtctatt gatgacgaat ggttgaagaa ggaattcgaa cacatcggtg gtgtcattgg
     1261 tgccagagtt atttatgaaa gaggtaccga tagatctcgt ggttatggtt acgttgattt
     1321 tgaaaacaaa tcttatgctg aaaaggccat tcaagaaatg caaggtaagg aaattgatgg
     1381 tagaccaatc aactgtgata tgtccacaag caagccagct ggtaacaacg atcgtgccaa
     1441 gaaattcggt gataccccat ctgaaccatc tgacactttg ttcttgggta acttatcttt
     1501 caatgctgac agagacgcta ttttcgaatt attcgctaaa cacggtgaag ttgtttccgt
     1561 ccgtatccca acacatccag aaactgaaca accaaaaggt ttcggttatg ttcaattctc
     1621 caacatggag gacgccaaga aggctctaga cgctttacaa ggtgaataca ttgacaacag
     1681 accagttaga ttagacttct cttctccaag accaaacaac gatggtggtc gtggcggtag
     1741 ccgtggtttt ggtggtcgtg gcggtggtcg tggcggtaac cgtggattcg gtggtcgtgg
     1801 tggcgctcgc ggtggccgtg gcggtttcag accatctggt tctggtgcta atactgctcc
     1861 attgggcaga tcaagaaata ccgcttcttt cgctggttca aagaaaacat ttgataaccc
     1921 agctttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt gttgcaacga
     1981 acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata tcccctatag
     2041 tgagtcgtat tacatggtca tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct
     2101 ctgatgttac attgcacaag ataaaataat atcatcatga acaataaaac tgtctgctta
     2161 cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cgaggccgcg
     2221 attaaattcc aacatggatg ctgatttata tgggtataaa tgggctcgcg ataatgtcgg
     2281 gcaatcaggt gcgacaatct atcgcttgta tgggaagccc gatgcgccag agttgtttct
     2341 gaaacatggc aaaggtagcg ttgccaatga tgttacagat gagatggtca gactaaactg
     2401 gctgacggaa tttatgcctc ttccgaccat caagcatttt atccgtactc ctgatgatgc
     2461 atggttactc accactgcga tccccggaaa aacagcattc caggtattag aagaatatcc
     2521 tgattcaggt gaaaatattg ttgatgcgct ggcagtgttc ctgcgccggt tgcattcgat
     2581 tcctgtttgt aattgtcctt ttaacagcga tcgcgtattt cgtctcgctc aggcgcaatc
     2641 acgaatgaat aacggtttgg ttgatgcgag tgattttgat gacgagcgta atggctggcc
     2701 tgttgaacaa gtctggaaag aaatgcataa acttttgcca ttctcaccgg attcagtcgt
     2761 cactcatggt gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg
     2821 tattgatgtt ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa
     2881 ctgcctcggt gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga
     2941 taatcctgat atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga
     3001 attggttaat tggttgtaac actggcagag cattacgctg acttgacggg acggcgcaag
     3061 ctcatgacca aaatccctta acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt
     3121 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     3181 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     3241 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     3301 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     3361 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     3421 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     3481 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     3541 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     3601 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     3661 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     3721 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     3781 tgctcacatg tt
//
