LOCUS       sequence-115941-         764 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2
            gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Paix A, Wang Y, Smith HE, Lee CY, Calidas D, Lu T, Smith J, Schmidt 
            H, Krause MW, Seydoux G
  TITLE     Scalable and versatile genome editing using linear DNAs with 
            microhomology to Cas9 Sites in Caenorhabditis elegans.
  JOURNAL   Genetics. 2014 Dec;198(4):1347-56. doi: 10.1534/genetics.114.170423.
            Epub 2014 Sep 23.
  PUBMED    25249454
REFERENCE   2  (bases 1 to 764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.114.170423"; journalName: "Genetics"; date: 
            "2014-12"; volume: "198"; issue: "4"; pages: "1347-56"
COMMENT     Sequence Label: AP54-5
FEATURES             Location/Qualifiers
     source          1..764
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(52..74)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_RNA        complement(346..421)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
       61 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
      121 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
      181 agagcagatt gcactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
      241 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg
      301 atcggtgcgg gcctcttcgc tattacgcca gaaatttcac aaaaagcacc gactcggtgc
      361 cactttttca agttgataac ggactagcct tattttaact tgctatttct agctctaaaa
      421 ctaatcatcg ccgtgactac caagacatct cgcaatagga ggtgtttata taggggcaac
      481 acaagaggcg gcagacatct cgtgagtttg acagagtagt ataaagagtg tgtgtgagag
      541 ggtgtaggca tttcataaca tattttttgt ggaataattt tggaaaaaag gtgttttttt
      601 aataatagct tttataagag tttctcaaaa attgcatgct ttttttgaaa aggttgacaa
      661 attatagtag tactaaagtt gctattttca ggtctcagca gaaaattcac aactaaaaat
      721 aaaatttgca aaacagagtg tgcgccttta aggtgtacag tttt
//
