LOCUS       sequence-115955-         663 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of amiR-eGFP from the broadly active CAG 
            promoter/enhancer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Miura H, Inoko H, Tanaka M, Nakaoka H, Kimura M, Gurumurthy CB, Sato
            M, Ohtsuka M
  TITLE     Assessment of Artificial MiRNA Architectures for Higher Knockdown 
            Efficiencies without the Undesired Effects in Mice.
  JOURNAL   PLoS One. 2015 Aug 18;10(8):e0135919. doi: 
            10.1371/journal.pone.0135919. eCollection 2015.
  PUBMED    26285215
REFERENCE   2  (bases 1 to 663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-08-18"; volume: "10(8):e0135919. doi"; pages: " 
            10.1371/journal.pone.0135919. eCollection 2015"
COMMENT     Sequence Label: pARC (pKD-G3)
FEATURES             Location/Qualifiers
     source          1..663
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           complement(87..128)
                     /label=3' miR-155
                     /note="sequence downstream of the precursor of mouse 
                     miR-155 microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(192..218)
                     /label=5' miR-155
                     /note="sequence upstream of the precursor of mouse miR-155 
                     microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(267..286)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(348..370)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 gccacaccag ccaccacctt ctgataggca gcctgcacct gaggagtgaa ttctgggtct
       61 agatatctcg agtgcggcca gatctgggcc atttgttcca tgtgagtgct agtaacaggc
      121 cttgtgtcct gatgaacttc agtcagcttg gtcagtcagt ggccaaaacc aagctgaccc
      181 tgaagttcat cagcatacag ccttcagcaa gcctccagga tccactggtc gactcactag
      241 aattctttgc caaaatgatg agacagcaca ataaccagca cgttgcccag gagctgtagg
      301 aaaaagaaga aggcatgaac atggttagca gaggctctag agccgccggt cacacgccag
      361 aagccgaacc ccgccctgcc ccgtcccccc cgaaggcagc cgtccccccg cggacagccc
      421 cgaggctgga gagggagaag gggacggcgg cgcggcgacg cacgaaggcc ctccccgccc
      481 atttccttcc tgccggcgcc gcaccgcttc gccccgcgcc cgctagaggg ggtgcggcgg
      541 cgcctcccag atttcggctc cgcacagatt tgggacaaag gaagtccctg cgccctctcg
      601 cacgattacc ataaaaggca atggctgcgg ctcgccgcgc ctcgacagcc gccggcgctc
      661 cgg
//
