LOCUS       sequence-115976-        1048 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Hesselson D, Anderson RM, Beinat M, Stainier DY
  TITLE     Distinct populations of quiescent and proliferative pancreatic 
            beta-cells identified by HOTcre mediated labeling.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Sep 1. 106(35):14896-901.
  PUBMED    19706417
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2009 Sep 1. 106(35):14896-901."
COMMENT     Sequence Label: hsp70l-loxP-mCherry-STOP-loxP-H2B-GFP_cryaa-ce...
FEATURES             Location/Qualifiers
     source          1..1048
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     28..48
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        30..48
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(677..698)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(938..957)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 cgcgtattac cctgttatcc ctagcgcgca attaaccctc actaaaggga acaaaagctg
       61 ggtaccatta atagtgtgca ttcagtgcag gatgttatgt atttattttc tgtagtgcaa
      121 actttttcaa agaattttcc agtctaagct tttcagtcaa gaaaaaaaaa aagcgattga
      181 ttatctgtga tatttaaaaa aattagctct taaatctggt gaaccaagtg gccacaacac
      241 attaaatact gacgttctac agccatgtag cttcatctgg tctggtttgt tttggcaggc
      301 acttattttc cggtgatagt tggccacaag agtattgtct gagccattca gtgctagact
      361 gtcattctca ggtcagagtc caccccgctg attctgctga caacactgtt cccaccatga
      421 gataatgcca ttccagagag atccatttgt aagcccctct ttctgcagca caggtatata
      481 accaggggtc tgcctccact aaggccggca cacatcattt ggggatcttt gtactgtact
      541 agcctctatt agcctcctac ttgcgttcag ttcacatctt ttgttcatca ggtgcaataa
      601 gttacagtaa ggagttacca ggtctgacaa catggtgagc aagggcgagg agctgttcac
      661 cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt
      721 gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac
      781 caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct ggggcgtgca
      841 gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc
      901 cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg
      961 cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga
     1021 cttcaaggag gacggcaaca tcctgggg
//
