LOCUS       sequence-115979-         589 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 589)
  AUTHORS   Hesselson D, Anderson RM, Beinat M, Stainier DY
  TITLE     Distinct populations of quiescent and proliferative pancreatic 
            beta-cells identified by HOTcre mediated labeling.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Sep 1. 106(35):14896-901.
  PUBMED    19706417
REFERENCE   2  (bases 1 to 589)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2009 Sep 1. 106(35):14896-901."
COMMENT     Sequence Label: hsp70l-loxP-mCherry-STOP-loxP-H2B-GFP_cryaa-ce...
FEATURES             Location/Qualifiers
     source          1..589
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     20..41
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(104..121)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    110..317
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    360..481
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(371..390)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     425..444
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
       61 tcggcatgga cgagctgtac aagtaaagcg gcccgctgat cagcctcgac tgtgccttct
      121 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
      181 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
      241 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
      301 agcaggcatg ctggggagag atcgccgcca ccgcggtggg taccgggccc cccctcgatt
      361 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
      421 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
      481 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt
      541 ttaaagcaag taaaacctct acaaatgtgg tatggctgat tatgatcta
//
