LOCUS       sequence-116008-        1012 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Hesselson D, Anderson RM, Beinat M, Stainier DY
  TITLE     Distinct populations of quiescent and proliferative pancreatic 
            beta-cells identified by HOTcre mediated labeling.
  JOURNAL   Proc Natl Acad Sci U S A. 2009 Sep 1. 106(35):14896-901.
  PUBMED    19706417
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2009 Sep 1. 106(35):14896-901."
COMMENT     Sequence Label: hsp70l-loxP-mCherry-STOP-loxP-H2B-GFP_cryaa-ce...
FEATURES             Location/Qualifiers
     source          1..1012
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    388..522
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(425..444)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     479..498
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    complement(577..610)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt tacaccacaa
       61 aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa atattctgta acctttataa
      121 gtaggcataa cagttataat cataacatac tgttttttct tactccacac aggcatagag
      181 tgtctgctat taataactat gctcaaaaat tgtgtacctt tagcttttta atttgtaaag
      241 gggttaataa ggaatatttg atgtatagtg ccttgactag agatcataat cagccatacc
      301 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
      361 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
      421 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
      481 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tctgacatgg taagtaagct
      541 tatccccgga gcttgggctg caggtcgagg gacctaataa cttcgtatag catacattat
      601 acgaagttat attaagggtt ccggatcgat cccctcgacg gatccaccgg tggccggcca
      661 ggcgcgccac catgccagag ccagcgaagt ctgctcccgc cccgaaaaag ggctccaaga
      721 aggcggtgac taaggcgcag aagaaaggcg gcaagaagcg caagcgcagc cgcaaggaga
      781 gctattccat ctatgtgtac aaggttctga agcaggtcca ccctgacacc ggcatttcgt
      841 ccaaggccat gggcatcatg aattcgtttg tgaacgacat tttcgagcgc atcgcaggtg
      901 aggcttcccg cctggcgcat tacaacaagc gctcgaccat cacctccagg gagatccaga
      961 cggccgtgcg cctgctgctg cctggggagt tggccaagca cgccgtgtcc ga
//
