LOCUS       sequence-116113-         691 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of His-tagged roGFP2-Orp1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Gutscher M, Sobotta MC, Wabnitz GH, Ballikaya S, Meyer AJ, Samstag 
            Y, Dick TP
  TITLE     Proximity-based protein thiol oxidation by H2O2-scavenging 
            peroxidases.
  JOURNAL   J Biol Chem. 2009 Nov 13;284(46):31532-40. doi: 
            10.1074/jbc.M109.059246. Epub 2009 Sep 15.
  PUBMED    19755417
REFERENCE   2  (bases 1 to 691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M109.059246"; journalName: "J Biol Chem"; date: 
            "2009-11-13- 13"; volume: "284"; issue: "46"; pages: "31532-40"
COMMENT     Sequence Label: pQE-60 roGFP2-Orp1-His
FEATURES             Location/Qualifiers
     source          1..691
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(44..61)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ctggatctat caacaggagt ccaagctcag ctaattaagc ttagtgatgg tgatggtgat
       61 gagatctttc cacctctttc aaaagttctt cgatggtttc ggacaacgaa gaaggtttgg
      121 ttagtgaaga gtatctttcg tacactttac cctttttatc gactaagaat ttttcaaaat
      181 tccatttgat acctctcaag cccaacatac cggatttttg gctcttcaaa aacttgtaaa
      241 cagggtcctc attgccacca ttaacgtcaa tttttttcat aatggggaaa gtcacgccat
      301 agttcagttg gcagaactgg gcaatttctt catcagagcc aggttcttgg tggccaaact
      361 ggttgcatgg gaacccgatg atggtaaatc cttcgtcctt ataacgtttg tacaaggcct
      421 ctagttcttt gtattgagga gtgaatccac atttggaggc aacattaacg ataagcacca
      481 cttttccctt taattggtcg aaggggaatg gttggccttt cttgtcaaca ggtgctagct
      541 tatagaattc tgagatatcg aattctcctc ctgatcctcc tcctcctgat cctcctcctc
      601 ctgatcctcc tccacctgaa ccaccaccac ctgaaccacc accacctgaa ccaccactag
      661 tcttgtacag ctcgtccatg ccgagagtga t
//
