LOCUS       sequence-116175-         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  AUTHORS   O'Gorman S, Dagenais NA, Qian M, Marchuk Y
  TITLE     Protamine-Cre recombinase transgenes efficiently recombine target 
            sequences in the male germ line of mice, but not in embryonic stem 
            cells.
  JOURNAL   Proc Natl Acad Sci U S A. 1997 Dec 23;94(26):14602-7.
  PUBMED    9405659
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "1997-12-23"; volume: "94(26)"; pages: 
            "14602-7"
COMMENT     Sequence Label: pOG231
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     333..352
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             392..412
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttcta ggaagatctt cctagaattc gctgtctgcg agggccagct gttggggtga
      121 gtactccctc tcaaaagcgg gcatgacttc tgcgctaaga ttgtcagttt ccaaaaacga
      181 ggaggatttg atattcacct ggcccgcggt gatgcctttg agggtggccg cgtccatctg
      241 gtcagaaaag acaatctttt tgttgtcaag cttgaggtgt ggcaggcttg agatcgatct
      301 ggccatacac ttgagtgaca atgacatcca ctttgccttt ctctccacag gtgtccactc
      361 ccaggtccaa ccgggatctc ccggggccat gcccaagaag aagaggaagg tgtccaattt
      421 actgaccgta caccaaaatt tgcctgcatt accggtcgat gcaacgagtg atgaggttcg
      481 caagaacctg atggacatgt tcagggatcg ccaggcgttt tctgagcata cctggaaaat
      541 gcttctgtcc gtttgccggt cgtgggcggc atggtgcaag ttgaataacc ggaaatggtt
      601 tcccgcagaa cctgaagatg ttcgcgatta tcttctatat cttcaggcgc gcggtctggc
      661 agtaaaaact atccagcaac atttgggcca gctaaacatg cttcatcgtc ggtccgggct
      721 gccacgacca agtgacagca atgctgtttc actggttatg cggcggatcc gaaaagaaaa
      781 cgttgatgcc ggtgaacgtg caaa
//
