LOCUS       sequence-116192-         809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of cytosolic Grx1-roGFP2 (retroviral vector).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Gutscher M, Pauleau AL, Marty L, Brach T, Wabnitz GH, Samstag Y, 
            Meyer AJ, Dick TP
  TITLE     Real-time imaging of the intracellular glutathione redox potential.
  JOURNAL   Nat Methods. 2008 Jun;5(6):553-9. doi: 10.1038/nmeth.1212. Epub 2008
            May 11.
  PUBMED    18469822
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1212"; journalName: "Nat Methods"; date: "2008-06"; 
            volume: "5"; issue: "6"; pages: "553-9"
COMMENT     Sequence Label: pLPCX cyto Grx1-roGFP2
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(503..524)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(764..783)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgctaccgg actcagatct atggctcaag agtttgtgaa
       61 ctgcaaaatc cagcctggga aggtggttgt gttcatcaag cccacctgcc cgtactgcag
      121 gagggcccaa gagatcctca gtcaattgcc catcaaacaa gggcttctgg aatttgtcga
      181 tatcacagcc accaaccaca ctaacgagat tcaagattat ttgcaacagc tcacgggagc
      241 aagaacggtg cctcgagtct ttattggtaa agattgtata ggcggatgca gtgatctagt
      301 ctctttgcaa cagagtgggg aactgctgac gcggctaaag cagattggag ctctgcagac
      361 tagtggtggt tcaggtggtg gtggttcagg tggtggtggt tcaggtggag gaggatcagg
      421 aggaggagga tcaggaggag gaggatcagg aggagaattc gtgagcaagg gcgaggagct
      481 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
      541 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
      601 ctccaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
      661 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
      721 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
      781 gacccgcgcc gaggtgaagt tcgagggcg
//
