LOCUS       sequence-116223-         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FRET biosensor Destination vector for E. coli expression. Encodes a 
            FRET pair for fusion with ligand binding domain of interest..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Jones AM, Danielson JA, Manojkumar SN, Lanquar V, Grossmann G, 
            Frommer WB
  TITLE     Abscisic acid dynamics in roots detected with genetically encoded 
            FRET sensors.
  JOURNAL   eLife. 2014 Apr 15;3:e01741. doi: 10.7554/eLife.01741.
  PUBMED    24737862
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2014-04-15"; volume: "3:e01741. doi"; pages: " 
            10.7554/eLife.01741"
COMMENT     Sequence Label: pFLIP39
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             35..52
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             56..88
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(92..100,101..115)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     protein_bind    831..851
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 actttaagaa ggagatatac atatgcgggg ttctcatcat catcatcatc atggtatggc
       61 tagcatgact ggtggacagc aaatgggtcg ggatctgtac gacgatgacg ataaggatcc
      121 gggcctcgag gtttctaaag gtgaagaatt gtttacgggc gtcgtcccga tcctcgtgga
      181 actcgacggg gatgttaacg ggcataagtt ttcggtcagc ggggaagggg agggggacgc
      241 gacgtatggg aagctcactc tcaagctgat ctgtacgacg gggaaactcc cggtcccgtg
      301 gccgacgctg gtcacgacgc tgggatacgg gctccaatgc tttgcgaggt atccggacca
      361 catgaaacag catgactttt tcaaatcggc gatgccggag ggatacgtgc aggaacggac
      421 gatctttttc aaagacgatg ggaactataa gacgcgggcg gaagtcaagt ttgaagggga
      481 cacgctcgtc aaccggatcg aactcaaggg gattgacttc aaagaggatg ggaacatact
      541 cggccataag ctcgaataca attacaactc gcataacgta tacatcaccg cggataagca
      601 aaagaacggg atcaaagcca atttcaaaat ccggcataac atagaggatg ggggggtcca
      661 actggcggat cactatcagc aaaacacgcc gataggggat gggccggtcc tcctcccgga
      721 taaccattac ctctcgtacc aaagcgcgct cttcaaggac ccgaatgaga aacgggacca
      781 catggttctc ctggagttcc tcacggcggc gggcataggt accgatatca caagtttgta
      841 caaaaaagct g
//
