LOCUS       sequence-116262-         663 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of amiR-eGFP from the broadly active CAG 
            promoter/enhancer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Miura H, Inoko H, Tanaka M, Nakaoka H, Kimura M, Gurumurthy CB, Sato
            M, Ohtsuka M
  TITLE     Assessment of Artificial MiRNA Architectures for Higher Knockdown 
            Efficiencies without the Undesired Effects in Mice.
  JOURNAL   PLoS One. 2015 Aug 18;10(8):e0135919. doi: 
            10.1371/journal.pone.0135919. eCollection 2015.
  PUBMED    26285215
REFERENCE   2  (bases 1 to 663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-08-18"; volume: "10(8):e0135919. doi"; pages: " 
            10.1371/journal.pone.0135919. eCollection 2015"
COMMENT     Sequence Label: pATI (pKD-G7)
FEATURES             Location/Qualifiers
     source          1..663
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           complement(85..126)
                     /label=3' miR-155
                     /note="sequence downstream of the precursor of mouse 
                     miR-155 microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(190..216)
                     /label=5' miR-155
                     /note="sequence upstream of the precursor of mouse miR-155 
                     microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(223..264)
                     /label=3' miR-155
                     /note="sequence downstream of the precursor of mouse 
                     miR-155 microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(328..354)
                     /label=5' miR-155
                     /note="sequence upstream of the precursor of mouse miR-155 
                     microRNA (Uva et al., 2013)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(403..422)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(484..506)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 cacaccagcc accaccttct gataggcagc ctgcacctga ggagtgaatt ctgggtctag
       61 atatctcgag tgcggccaga tctgggccat ttgttccatg tgagtgctag taacaggcct
      121 tgtgtcctgt gtagttgtac cagcttgtgt cagtcagtgg ccaaaacaca agctggagta
      181 caactacaca gcatacagcc ttcagcaagc ctccaggatc tgggccattt gttccatgtg
      241 agtgctagta acaggccttg tgtcctgatg aacttcagtc agcttggtca gtcagtggcc
      301 aaaaccaagc tgaccctgaa gttcatcagc atacagcctt cagcaagcct ccaggatcca
      361 ctggtcgact cactagaatt ctttgccaaa atgatgagac agcacaataa ccagcacgtt
      421 gcccaggagc tgtaggaaaa agaagaaggc atgaacatgg ttagcagagg ctctagagcc
      481 gccggtcaca cgccagaagc cgaaccccgc cctgccccgt cccccccgaa ggcagccgtc
      541 cccccgcgga cagccccgag gctggagagg gagaagggga cggcggcgcg gcgacgcacg
      601 aaggccctcc ccgcccattt ccttcctgcc ggcgccgcac cgcttcgccc cgcgcccgct
      661 aga
//
